| 谭小方,金 华,徐 丽,施蔚虹,李海波,丁家怡.人早期胚胎解冻后氨基酸代谢变化的临床分析[J].,2017,17(5):933-936 |
| 人早期胚胎解冻后氨基酸代谢变化的临床分析 |
| Analysis on Changes in Amino Acid Metabolism after Human Early Embryos |
| 投稿时间：2016-05-15 修订日期：2016-06-25 |
| DOI：10.13241/j.cnki.pmb.2017.05.035 |
| 中文关键词: 冻融胚胎移植 移植时机 氨基酸代谢 高效液相色谱法 |
| 英文关键词: Freezing and thawing embryo transfer Transplant time Amino acid metabolism High performance liquid chromatography |
| 基金项目:江苏省妇幼保健科研项目（F201347）；南通市科技计划项目（HS2013012） |
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| 中文摘要: |
| 摘要 目的：分析人早期胚胎解冻后的氨基酸代谢变化，以明确冻融胚胎解冻后的最佳移植时机。方法：收集2013年1月-2014年1月本中心24例经体外受精-胚胎移植治疗患者取卵后第3日6~8细胞废弃胚胎，采用囊胚培养液微滴对其培养，培养2h后用玻璃化冷冻保存，于冷冻前2h、解冻后各时段分别收集15 μL胚胎培养微滴，以同一培养皿中未进行胚胎培养的培养液为对照组。通过高效液相色谱法检测标本在不同时段（0.5、1、2、4、6、24 h）的氨基酸浓度变化。结果：对照组解冻后不同时间点赖氨酸、色氨酸、组氨酸与谷氨酰胺浓度与与解冻前比较，差异具均有统计学意义（P＜0.05），其余16种氨基酸解冻前后浓度无明显变化，差异均无统计学意义（P＞0.05）。胚胎培养液解冻后1 h，胚胎培养液中的氨基酸浓度均明显高于对照组，差异具有统计学意义（P＜0.05），氨基转换量明显高于解冻前、解冻后0.5 h、4 h、6 h，差异具有统计学意义（P＜0.05）。结论：人早期胚胎在解冻后约0.5h就已开始恢复代谢状态，已开始进行氨基酸代谢，且氨基酸代谢水平与冷冻前基本相同。 |
| 英文摘要: |
| ABSTRACT Objective: To detection and analysis on changes in amino acid metabolism after human early embryos, in order to de- fine the best opportunity for the transplantation of frozen thawed embryos. Methods: Selected 24 cases of third days after fertilization and embryo transfer treatment in patients with 6~8 cell abandoned embryos who treated in the center from January 2013 to January 2014, the blastocyst culture solution microdroplet was applied to culture such embryos,vitrification cryopreservation was taken after 2 hours of cul- ture, 15μL of embryo culture droplets were respectively collected at time frames of 2 h before freezing and after thawing, embryos in the culture solution in the same petri dish bypassing embryo culture were selected into the control group. The changes in amino acid concen- tration were detected at different times via performance(0.5, 1, 2, 4, 6, 24 h)by high performance liquid chromatography. Results: In the control group, the concentration of lysine, tryptophan, histidine and glutamine after thawing at the different time points were statistically significant compared with before thawing(P<0.05), the remaining 16 amino acids before and after thawing concentration had no signifi- cant changes, the difference were not statistically significant(P>0.05), 1 h after thawing,the concentrations of amino acids in embryo cul- ture medium were significantly higher than those in the control group, the differences were statistically significant(P<0.05), thawing amino conversion were significantly higher than that of before thawing, 0.5 h, 4 h, and 6 h, the differences were statistically significant(P<0.05). Conclusion: Human early embryos have begun to recover about 0.5 h after thawing, amino acid metabolism has been started, and its metabolism of amino acid is basic same to that before thawing. |
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