| 田海文 刘斌 李艳春 柳洁 饶利兵 陈峡.急性酒精中毒合并中度创伤性脑损伤对大鼠海马NOS2 表达和学习记忆的影响[J].,2017,17(3):438-442 |
| 急性酒精中毒合并中度创伤性脑损伤对大鼠海马NOS2 表达和学习记忆的影响 |
| Moderate Traumatic Brain Injury Following Acute AlcoholismAffect RatsStudy-Memory and NOS2 Expression in Hippocampus |
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| DOI： |
| 中文关键词: 创伤性脑损伤 神经元型一氧化氮合酶 水迷宫 |
| 英文关键词: TBI NOS2 Morris-Maze |
| 基金项目:湖南省教育厅科研基金项目（11C1013）；湖南医药学院校级科研项目（2010KY06） |
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| 中文摘要: |
| 目的：探讨急性酒精中毒合并中度创伤性脑损伤对大鼠海马NOS2 表达和学习记忆的影响。方法：健康成年雄性SD 大鼠
96只，水迷宫训练3 天后分为4 组：生理盐水组（N 组）、急性酒精中毒组（A 组）、中度创伤性脑损伤组（T 组）和急性酒精中毒合
并中度创伤性脑损伤（AT 组）。腹腔单注射25%酒精（2.5g/kg），2 h后以重物自由落体击打大鼠头部建立动物模型，存活1、3、5、7、
14 天。免疫组化方法检测海马CA1 区NOS2 表达，水迷宫检测大鼠学习记忆。结果：NOS2 免疫组化染色发现各实验组阳性细胞
数均高于N 组。术后1天T 组比AT 组表达显著增高（P<0.01）；术后5 天AT 组比T 组表达增高（P<0.05）；术后14 天AT 组比T
组表达显著增高（P<0.05）。水迷宫实验测潜伏期，术后1 天AT组比T组延长（P<0.05），术后3 天AT 组比T 组缩短(P<0.05)，术
后14 天AT组比T组显著延长(P<0.01)。结论：大鼠急性酒精中毒合并颅脑外伤后晚期，潜伏期延长，空间位置学习与记忆能力显
著下降；在海马CA1 区NOS2 表达阳性细胞增多，为继发性脑损伤致其表达上调，是酒精急性中毒合并中度颅脑外伤预后欠佳的
原因之一。 |
| 英文摘要: |
| Objective:To investigate the spatial memory and the expression of hippocampal NOS2 in rat model of acute alcohol
intoxication combined with TBI.Methods:After practice in water maze, 96 male SD rats were randomly divided into four groups: N
group (saline injection), A group (alcohol injection), T group (traumatic brain injury and saline injection) and AT group (traumatic brain
injury and alcohol injection). The animal model of acute alcohol intoxication with TBI was established by hitting rat forehead vertically 2
hours following injecting of 25%alcohol 2.5 g/kg intraperitoneally. Animals of each group sacrificed at 1, 3, 5, 7, 14 days after behavior
tests respectively. The spatial memory was tested by Morris-maze. The expression of NOS2 in hippocampus neurons was detected by immunohistochemistry
method.Results:All experimental groups exhibit higher expression of NOS2 than that of N control group. NOS2 expression
in T group was higher than in AT group at the 1st day after surgery (P<0.01). NOS2 expression in AT group was higher than that
of T group from 5th day to14th day after surgery（P<0.05）. In water maze tests, latency in T group was shorter than that in AT group (P<0.
05) in the1st and the 14th day. However, in the 3rd day, latency in NT group was longer than in AT group (P<0.05).Conclusion:Acute alcohol
intoxication with advanced TBI had long latency in finding the platform, suggesting that capacity of spatial learning and memory
was impaired. The increased expression of NOS2 in rat CA1 neurons area of hippocampus may contribute to secondary brain injury, and
may be one of the reasons for unfavourable prognosis in later stage. |
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