| 李西安 魏迪 王锁刚 闫飞 叶永利 袁建林△.羟苯磺酸钙通过抑制Ⅰ型胶原表达减轻肾间质纤维化[J].,2017,17(3):405-409 |
| 羟苯磺酸钙通过抑制Ⅰ型胶原表达减轻肾间质纤维化 |
| CalciumDobesilate Alleviate Renal Interstitial Fibrosis by Inhibition of Collagen I |
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| DOI： |
| 中文关键词: 羟苯磺酸钙 肾间质纤维化 Ⅰ型胶原 |
| 英文关键词: Calciumdobesilate Renal interstitial fibrosis Collagen I |
| 基金项目:国家自然科学基金面上项目（30872583）；陕西省协同创新计划项目（2015XT-53） |
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| 中文摘要: |
| 目的：研究羟苯磺酸钙对小鼠肾间质纤维化、Ⅰ型胶原表达的影响。方法：将C57 小鼠随机分为假手术组（Sham组，n=4）、肾
间质纤维化模型组（UUO 组，n=5）及羟苯磺酸钙治疗组（CDT组，n=4）；采用单侧输尿管梗阻制备肾间质纤维化模型，CDT 组给
予羟苯磺酸钙灌胃、Sham组和UUO组给予双蒸水灌胃；采用HE 染色、Masson 染色、免疫组化、实时定量PCR 以及蛋白免疫印
迹观察单侧输尿管梗阻术后14 d小鼠术侧肾脏的肾间质纤维化程度和Ⅰ型胶原表达情况。结果：与Sham组比较，UUO 组小鼠术
后14 d术侧肾脏肾发生显著肾间质纤维化，Ⅰ型胶原表达显著增强（Ⅰ型胶原基因相对表达量：Sham 组：1.00000，UUO 组：
114.92289，P<0.0001）。与UUO 组比较，CDT 组小鼠术后14 d术侧肾间质纤维化程度显著减轻，Ⅰ型胶原表达显著减弱（Ⅰ型胶
原基因相对表达量：UUO组：114.92289，CDT 组：45.33516，P<0.005）。结论：羟苯磺酸钙通过抑制小鼠肾间质Ⅰ型胶原表达从而
减轻单侧输尿管结扎小鼠肾间质纤维化。 |
| 英文摘要: |
| Objective:To investigate the effect of calcium dobesilate on the expression of collagen I in renal interstitial fibrosis in
ice.Methods:C57 mice were randomly divided into sham operation group (Sham group, n=4), renal interstitial fibrosis model group
(UUO group, n=5) and calciumdobesilate treatment group (CDT group, n=4), preparation of renal interstitial fibrosis model by unilateral
ureteral obstruction (UUO), CDT Group was treated with calcium dobesilate orally, Sham group and UUO group were given double
distilled water , HE staining, Masson staining, immunohistochemistry, real-time quantitative PCR and western blot was detected after 14
days of UUO in mice to observe the renal interstitial fibrosis degree and the expression of collagen I.Results:Compared with the Sham
group, the renal interstitial fibrosis were significantly increased in the UUO group after operation 14 d, the expression of collagen I was
significantly enhanced (collagen I gene relative expression: Shamgroup: 1, UUO group: 114.92289, P<0.0001). Compared with the UUO
group, the degree of renal interstitial fibrosis in CDT group was relieved after operation 14 d, and the expression of collagen I was
decreased (collagen I gene relative expression: UUO group: 114.92289, CDT group: 45.33516, P<0.005).Conclusion:Calcium
dobesilate can reduce renal interstitial fibrosis in mice with UUO by inhibiting the expression of collagen I in mouse renal interstitial. |
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