| 贺 帅,吴彦萍,蔡湘仪,陈红波,王丽君,黄来强.姜黄素对宫颈癌细胞生长与增殖的影响研究[J].,2017,17(1):7-10 |
| 姜黄素对宫颈癌细胞生长与增殖的影响研究 |
| Study on the Effect of Curcumin on Growth and Proliferation of HeLa Cell |
| 投稿时间：2016-04-30 修订日期：2016-05-24 |
| DOI：10.13241/j.cnki.pmb.2017.01.002 |
| 中文关键词: 姜黄素 上游结合因子 核仁转录 聚合酶链式反应 |
| 英文关键词: Curcumin Upstream binding factor (UBF) Nucleolus transcription Q-PCR |
| 基金项目:深圳市重点实验室计划以及深圳市科技项目（JCYJ20130402145002438, ZDSYS20140509172959975, GJHZ20140416153844269, JCYJ20140418112611757） |
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| 中文摘要: |
| 摘要 目的：探究姜黄素（Curcumin）对宫颈癌细胞HeLa生长与增殖的影响及其作用机制。方法：体外培养HeLa细胞，以0 μmol?L-1、5 μmol?L-1、10 μmol?L-1和20 μmol?L-1等不同浓度的姜黄素处理HeLa细胞；通过MTT和平板克隆实验，研究姜黄素对HeLa细胞生长与增殖的影响；通过免疫荧光共聚焦显微镜技术，研究姜黄素与上游结合因子（Upstream binding factor，UBF）在细胞中的定位情况；通过实时定量聚合酶链式反应（Q-PCR）实验，研究姜黄素对HeLa细胞核仁rDNA转录的影响；利用Western blot技术，研究姜黄素对HeLa细胞中UBF磷酸化的影响。结果：姜黄素对HeLa细胞有明显的生长抑制作用，并呈现出剂量依赖关系；姜黄素降低rDNA的转录水平，下调UBF磷酸化的量，且和UBF在核仁中存在共定位现象。结论：rDNA转录水平的降低和p-UBF的下调可能是姜黄素抑制HeLa细胞生长和增殖的作用途径之一。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of curcumin on growth and proliferation of human cervical cancer HeLa cell, as well as the mechanism. Methods: HeLa cell was treated with different concentrations of curcumin, 0 μmol?L-1, 5 μmol?L-1, 10 μmol?L-1, 20 μmol?L-1, in vitro. MTT and plate cloning experiments were used to study the effect of curcumin on growth of HeLa cell. Immune fluo- rescence confocal experiment was used to study the localization of curcumin and UBF in the cell. The real-time polymerase reaction ex- periment was used to detect the nucleolus transcription of HeLa cell. Western blot was used to study the level of phosphorylation of UBF. Results: Curcumin has an inhibitory effect on the growth of HeLa cell in a dose-dependent manner. Moreover, curcumin is co-localized in the nucleolus with UBF and could down-regulate the level of nucleolus transcription and the level of phosphorylation of UBF. Conclusion: The inhibitory effect may be achieved through the down-regulation of nucleolus transcription and the level of p-UBF. |
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