| 霍爱鑫 巴燕娜 吴振彪 崔轶霞 贾筠.miR-21 和miR-155在系统性红斑狼疮患者外周血B 细胞中的表达及意义[J].,2015,15(35):6948-6951 |
| miR-21 和miR-155在系统性红斑狼疮患者外周血B 细胞中的表达及意义 |
| The Expressions and Significances of miR-21 and miR-155 in PeripheralBlood B Cells of Patients with Systemic Lupus Erythematosus |
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| DOI： |
| 中文关键词: 系统性红斑狼疮 外周血B 细胞 miR-21 miR-155 骨形成蛋白-2 骨形成蛋白受体Ⅱ |
| 英文关键词: Systemic lupus erythematosus Peripheral blood B cells miR-21 miR-155 Bone morphogenetic protein-2 (BMP-2) Bone morphogenetic protein receptor Ⅱ (BMPRⅡ) |
| 基金项目:陕西省自然科学基金项目(2013JQ4030) |
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| 中文摘要: |
| 目的：探讨miR-21 和miR-155 在系统性红斑狼疮患者外周血B 细胞中的表达及意义。方法：将93 例系统性红斑狼疮(SLE)
患者根据SLEDAL评分分为轻度活动组(n=49)和中重度活动组(n=44)，并选择40 例健康者作为对照组，采用实时荧光定量PCR
技术检测其外周血B细胞中miR-21、miR-155、骨形成蛋白-2(BMP-2)和骨形成蛋白受体Ⅱ(BMPRⅡ)的表达。结果：SLE 患者外
周血B 细胞中miR-21 和miR-155 相对表达量均高于对照组(P<0.05)，中重度活动组患者外周血B 细胞中miR-21 和miR-155 相
对表达量均高于轻度活动组和对照组，且轻度活动组高于对照组，差异均有统计学意义(P<0.05)；SLE 患者外周血B 细胞中
BMP-2 mRNA 和BMPRⅡ mRNA 相对表达量均高于对照组(P<0.05)，中重度活动组患者外周血B 细胞中BMP-2 mRNA和BMPR
Ⅱ mRNA 相对表达量均高于轻度活动组和对照组，且轻度活动组高于对照组，差异均有统计学意义(P<0.05)。Pearson相关分析显
示SLE 患者外周血B 细胞中miR-21 和miR-155 相对表达量与BMP-2 mRNA和BMPRⅡ mRNA均呈正相关(r=0.292、0.365 和
0.481、0.279，P<0.05)，SLE 患者外周血B细胞中miR-21 和miR-155 相对表达量均与补体C3 和补体C4 水平呈显著负相关(r=-0.
570、-0.289 和-0.751、-0.308，P<0.05)。结论：miR-21 和miR-155 在SLE 患者外周血B细胞中呈高表达，可能通过BMP信号通路
异常活化而发挥作用，且与补体C3 和C4 呈负相关，有望作为监控SLE 患者病情的参考指标。 |
| 英文摘要: |
| Objective:To investigate the expressions and significances of miR-21 and miR-155 in peripheral blood B cells of
patients with systemic lupus erythematosus(SLE).Methods:93 cases of patients with SLE were divided into mild active group (n=49) and
moderate and severe active group (n=44) according to SLEDAL scores. 40 healthy were selected as the control group. The expressions of
miR-21, miR-155, bone morphogenetic protein-2 (BMP-2) and bone morphogenetic protein receptor Ⅱ (BMPRⅡ) in peripheral blood B
cells were detected by using real-time quantitative PCR.Results:The relative expressions of miR-21 and miR-155 in peripheral blood B
cells of SLE patients were higher than the control group (P<0.05). The relative expressions of miR-21 and miR-155 in peripheral blood B
cells in the moderate and severe active group were higher than the mild active group and the control group, and the mild active group was
higher than the control group, the differences were statistically significant (P<0.05). The relative expressions of BMP-2 mRNA and
BMPRⅡ mRNA in peripheral blood B cells of SLE patients were higher than the control group (P<0.05). The relative expressions of
BMP-2 mRNA and BMPRⅡ mRNA in peripheral blood B cells in moderate and severe active group were higher than the mild active
group and the control group, and the mild active group was higher than the control group, the differences were statistically significant
(P<0.05). Pearson correlation analysis showed, the relative expressions of miR-21 and miR-155 in peripheral blood B cells of the SLE
patients were positively correlated with BMP-2 mRNA and BMPRⅡ mRNA (r=0.292, 0.365 and 0.481, 0.279, P<0.05), the relative
expressions of miR-21 and miR-155 in peripheral blood B cells of the SLE patients were negatively correlated with the levels of
complement C3 and C4 (r= -0.570, -0.289 and -0.751, -0.308, P<0.05).Conclusion:MiR-21 and miR-155 were highly expressed in the
peripheral blood B cells of SLE patients, which might play a role by the abnormal activation of BMP signaling and were negatively
correlated with the complement C3 and C4. they might expected to be indicators in monitoring the patient's condition. |
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