| 李暐 郭江睿 姚正盛 陈清 蔡心颖 张志强.扁蒴藤素对人鼻咽癌HNE2 细胞增殖的抑制作用研究[J].,2015,15(35):6826-6830 |
| 扁蒴藤素对人鼻咽癌HNE2 细胞增殖的抑制作用研究 |
| The Growth Inhibition Effects of Pristimerin on Human NasopharyngealCarcinoma Cell Line HNE2 |
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| DOI： |
| 中文关键词: 扁蒴藤素 鼻咽癌 酪氨酸激酶 热休克蛋白 |
| 英文关键词: Pristimerin Nasopharyngeal carcinoma Tyrosine kinase Heat shock protein |
| 基金项目:国家自然科学基金青年科学基金项目(81001453)；福建省自然科学基金青年基金项目(2010J05068)；福建省教育厅青年科技项目(JA10141) |
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| 中文摘要: |
| 目的：探讨扁蒴藤素对人鼻咽癌HNE2 细胞增殖的抑制作用，明确HSP70 在肿瘤发展过程中的抑制作用。方法：噻唑蓝法
（MTT）检测扁蒴藤素对HNE2 细胞生长抑制作用，流式细胞术检测扁蒴藤素诱导HNE2 细胞凋亡，免疫印迹法检测Caspase、
PARP、酪氨酸激酶、AKT 及Bcl-2 家族蛋白表达。结果：扁蒴藤素抑制HNE2 细胞的生长，促使Caspase 9，Caspase 3和PARP 蛋
白被切割，上调Bim 等促凋亡蛋白的表达，减少Bcl-xL等抗凋亡蛋白的表达，下调EGFR 等受体酪氨酸激酶, 抑制AKT 磷酸化，
上调热休克蛋白70（HSP70）的表达。用热休克反应抑制剂KNK437 抑制HSP70 的表达可以增强扁蒴藤素促进细胞凋亡的能力。
结论：扁蒴藤素通过下调受体酪氨酸激酶，激活caspase 介导的凋亡通路抑制鼻咽癌HNE2 细胞的增殖，抑制HSP70 的表达可增
强其抗肿瘤作用。 |
| 英文摘要: |
| Objective:To investigate the inhibitory effects of pristimerin on proliferation of human nasopharyngeal carcinoma
HNE2 cells, and the inhibition of HSP70 in tumor development.Methods:Methyl thiazolyl tetrazolium(MTT) assay was used to evaluate
the growth inhibition effects of pristimerin on HNE2 cells, flow cytometry was performed to detect pristimerin induced apoptosis in
HNE2 cells,Western blotting was used to detect the expression of Caspases, PARP, Bcl-2 family protein, tyrosine kinases and AKT.Results:Pristimerin can inhibit the growth of HNE2 cells, the Caspase 9, Caspase 3 and PARP protein was cut. Pristimerin down-regulated receptor
tyrosine kinases, modulated the expression of Bcl-2 family proteins, inhibited AKT phosphorylation, enhanced HSP70 expression and
finally triggered Caspases-mediated apoptotic signal transduction. The activity of inducing apoptosis of pristimerin could be enhanced
when combination of HSP70 inhibitor KNK437 with pristimerin augmented pristimerin induced apoptosis.Conclusion:Pristimerin can
induce Caspases-mediated apoptosis via modulating the expression of receptor tyrosine kinases and Bcl-2 family proteins, inhibiting AKT
phosphorylation. Inhibition of HSP70 can enhance the anti-tumor activities of pristimerin. |
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