| 王建材 张兴业 祝刚 李泉 高国栋 罗涛.阿司匹林可抑制吗啡引起的HT22 细胞Wnt通路的激活[J].,2016,16(33):6410-6413 |
| 阿司匹林可抑制吗啡引起的HT22 细胞Wnt通路的激活 |
| Morphine Induce the Activity of Wnt Signaling in HT22 Cells Whichcan be Inhibited by Aspirin |
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| DOI： |
| 中文关键词: HT22 细胞 wnt通路 β-catenin 成瘾 吗啡 |
| 英文关键词: HT22 cell Wnt signaling β-catenin Addiction Morphine |
| 基金项目:国家重点基础研究发展计划项目（973计划）（2011CB51000）；唐都医院科技创新发展基金重大国际合作项目（2013GJHZ001） |
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| 中文摘要: |
| 目的：研究阿司匹林对吗啡引起的Wnt通路的上调的影响。方法：在大鼠海马神经元元细胞系HT22 细胞上建立了慢性吗
啡成瘾模型，然后运用免疫荧光技术和Western blot技术检测wnt 通路中β-catenin 蛋白的表达变化变化，同时应用报告基因技术
检测Wnt通路下游转录因子的活性。结果：在慢性吗啡成瘾模型组，β-catenin 蛋白的表达显著增加，而在接受了6 h的阿司匹林
预处理的慢性吗啡成瘾模型组，β-catenin 蛋白的表达受到了显著的抑制。结论：阿司匹林可抑制吗啡引起的Wnt/茁-catenin 通路水
平的过表达。 |
| 英文摘要: |
| Objective:To analyse the role of aspirin in down-regulation the Wnt signaling which activated by morphine addiction.
Many studies have revealed that Wnt signaling activated in morphine addiction rats models.Methods:Many technologies such as Immunofluorescence,
western blot were carried out. We also used the Luciferase test to study the downstreamgene change in Wnt signaling
after morphine addiction with or without aspirin pretreatment.Results:Many technologies such as Immunofluorescence,
western blot were carried out. We also used the Luciferase test to study the downstreamgene change in Wnt signaling
after morphine addiction with or without aspirin pretreatment.Conclusion:Our study identified that aspirin can inhibited the Wnt signaling which activated after chronic morphine addiction and this may be important
in the cure of morphine addiction. |
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