| 古结乃特汗·拜克里木 韩玲 卫荣△ 巴燕·艾克海提 陈慕芝.湿热痹阻型与寒湿痹阻型类风湿关节炎患者细胞因子的差异[J].,2016,16(32):6298-6301 |
| 湿热痹阻型与寒湿痹阻型类风湿关节炎患者细胞因子的差异 |
| Difference of Cytokines in the Damp Heat Blockage Type andCold-dampness Blockage Type of Rheumatoid Arthritis |
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| DOI： |
| 中文关键词: 类风湿关节炎 湿热痹阻型 寒湿痹阻型 细胞因子 |
| 英文关键词: Rheumatoid arthritis Damp heat blockage type Cold-dampness blockage type Cytokines |
| 基金项目:新疆医科大学附属中医医院院级课题（ZYY201217） |
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| 中文摘要: |
| 目的：对比分析湿热痹阻型与寒湿痹阻型类风湿关节炎患者的相关细胞因子水平。方法：选取2012 年3 月至2015 年12 月
我院收治的47 例湿热痹阻型类风湿关节炎患者（湿热痹阻组）、43 例寒湿痹阻型类风湿关节炎患者（寒湿痹阻组）以及同期我院
体检科确诊的50 例健康体检者（对照组）作为研究对象。采用酶联免疫吸附试验（ELISA）双抗体夹心法检测所有研究对象的血清
基质金属蛋白酶-3（MMP-3）、金属蛋白酶抑制剂-1（TIMP-1）、肿瘤坏死因子-alpha（TNF-alpha）、白介素-1beta（IL-1茁），同时采用流式细胞
仪检测单核细胞表面CD147 分子的表达情况。结果：3 组研究对象血清MMP-3、TIMP-1、TNF-alpha、IL-1beta相比差异有统计学意义
（P＜0.05），对照组血清MMP-3 显著低于湿热痹阻组，TIMP-1、TNF-alpha、IL-1beta则显著低于湿热痹阻组与寒湿痹阻组（P＜0.05），寒
湿痹阻组血清MMP-3、TIMP-1 显著低于湿热痹阻组（P＜0.05），湿热痹阻组与寒湿痹阻组血清TNF-alpha、IL-1beta相比差异无统计学
意义（P＞0.05）。结论：湿热痹阻型与寒湿痹阻型类风湿关节炎患者的血清MMP-3、TIMP-1 及单核细胞表面CD147 存在着显著区别。 |
| 英文摘要: |
| Objective:To compare and analyze the difference of arthritis related cytokines between damp heat blockage type and
cold-dampness blockage type in the patients with rheumatoid arthritis.Methods:47 cases with rheumatoid arthritis of damp heat blockage
type (damp heat blockage type group), and 43 cases with rheumatoid arthritis of cold-dampness blockage type (cold-dampness blockage
group) and 50 cases of healthy person diagnosed by physical examination (control group) were selected as research objects. The level of
serum matrix metalloproteinase3 (MMP-3) and tissue inhibitor of metalloproteinase1 (TIMP-1), tumor necrosis factor alpha (TNF-alpha)
and interleukin-1 beta (IL-1beta) was examined by enzyme linked immunosorbent assay (ELISA) double antibody sandwich method, and
the expression of mononuclear cell surface CD147 was detected using the flow cytometry assay.Results:The difference of serum level of
MMP-3, TIMP-1, TNF-琢and IL-1茁among three groups was statistically significant (P<0.05). The serumlevel of MMP-3 in control group
was significantly lower than that of damp heat blockage group, and the level of TIMP-1, TNF-琢and IL-1茁was significantly lower than
that of damp heat blockage group and cold-dampness blockage group (P<0.05), and the serum level of MMP-3 and TIMP-1 was
significantly lower in cold-dampness blockage group than that of damp heat blockage group (P<0.05). The serum levels of TNF-alpha and
IL-1beta had no significantly statistical difference between damp heat blockage group and cold-dampness blockage group (P>0.05).Conclusion:The damp heat blockage type and cold-dampness blockage type rheumatoid arthritic inflammation in patients with serum
MMP-3, TIMP-1 and monocyte surface CD147 exist significant difference. |
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