| 马春华 韩莉莉 朱开春 王琳 玛依努尔·尼牙孜.HPV16 变异体在新疆地区宫颈癌及癌前病变中的分布研究[J].,2016,16(32):6244-6251 |
| HPV16 变异体在新疆地区宫颈癌及癌前病变中的分布研究 |
| Research on HPV16 Variants in Cervical Cancer and Precancerous Lesions in Xinjiang |
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| DOI： |
| 中文关键词: HPV16 变异体 宫颈癌 宫颈癌前病变 |
| 英文关键词: HPV16 variants Cervical Cancer Precancerous Lesions |
| 基金项目:国家自然科学基金项目（81450013，81360317）；新疆维吾尔自治区自然科学基金项目（2015211C208） |
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| 中文摘要: |
| 目的：研究新疆地区HPV16 E6、E7、LCR 基因突变情况，分析HPV16 变异体在宫颈癌及癌前病变发生发展中的作用。方法：
选择HPV16 阳性的宫颈癌及癌前病变患者，提取基因组DNA，利用PCR 扩增HPV16 DNA E6、E7 基因及LCR 区核苷酸片段，
正反向测序。与HPV16 基因序列分析比对，分析核苷酸突变位点。结果：E6 基因突变率为80.00%(92/115)主要突变位点T350G
(59.78%)、T178G(18.47%)；E7 突变率为54.78%(63/115)，主要突变位点A647G(33.33%)、T846C(26.98%)；LCR 突变率为23.48%
(27/115)，主要突变位点为C24T(74.07%)、C13T(25.92%)。维吾尔族T350G突变率较汉族妇女显著升高，而汉族A647G、T846C、
C24T 突变率显著高于维吾尔族，差异具有统计学意义(P＜0.05)。维吾尔族宫颈癌组T350G 突变率显著高于炎症组(P＜0.05)，且
随病变严重程度增加突变率上升，汉族T350G、A647G、T846C、C24T 突变率炎症组、宫颈病变组显著高于宫颈癌组(P＜0.05)，维
吾尔族C24T 突变率炎症组显著高于宫颈癌组(P＜0.05)，差异均具有统计学意义(P＜0.05)。结论：HPV16E6、E7 突变可能与宫颈
病变进展有关，T350G突变可能是维吾尔族宫颈癌高发的原因之一。 |
| 英文摘要: |
| Objective:To study E6, E7 and LCR gene mutation in Xinjiang; and analyze the effects of HPV16 variants in the
occurrence and development of cervical cancer and precancerous lesions.Methods:Genomic DNA was extracted in patients with HPV16
positive cervical cancer and precancerous lesions. HPV16 DNA E6 and E7 genes and nucleotide fragments of LCR region were carried
out the forward and reverse sequencing using PCR amplification. HPV16 pedigree distribution was confirmed via analyzing and
comparing the HPV16 gene sequencing to analyze the mutation sites of nucleotide.Results:E6 gene mutation rate was 80.00% (92/115)
and the main mutation sites were T350G (59.78%) and T178G (18.47%). E7 gene mutation rate was 54.78% (63/115) and the main
mutation sites were A647G (33.33%) and T846C (26.98%). LCR mutation rate was 23.48% (27/115) and the main mutation sites were
C24T (74.07%) and C13T (25.92%). Compared the Uyghur women with Han women, Uyghur T350G mutation rate was significantly
higher. Han A647G, T846C and C24T mutation rate were significantly higher than Uyghur (P＜0.05). T350G mutation in the Uyghur
cervical cancer group was significantly higher than that of inflammation group (P＜0.05). Moreover, the severity of mutation went up
along with lesions. Han T350G, A647G, T846C and C24T mutation rates in the inflammation group and cervical lesions group were
significantly higher than cervical cancer (P＜0.05). Uyghur C24T mutation rate in the inflammation group was significantly higher than
cervical cancer group (P＜0.05).Conclusion:HPV16E6 and E7 mutation might be related to cervical lesions. T350G mutation might be
the cause of the high incidence of cervical cancer in Uygur. |
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