| 徐旭 史剑 谈春业 刘锦波 何小舟.慢性移植性肾病大鼠脾脏中辅助性T细胞和B 细胞免疫状态的研究*[J].,2016,16(32):6220-6225 |
| 慢性移植性肾病大鼠脾脏中辅助性T细胞和B 细胞免疫状态的研究* |
| The Immune Status of Th and B Cells in the Rat Spleen during ChronicAllograft Nephropathy |
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| DOI： |
| 中文关键词: Th 细胞 B 细胞 慢性移植性肾病 |
| 英文关键词: Th cells B cells Chronic allograft nephropathy |
| 基金项目:国家自然科学基金面上项目（81273267，K112409514) |
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| 中文摘要: |
| 目的：检测慢性移植性肾病（CAN）大鼠脾脏中辅助性T细胞（Th）和B细胞特征性因子表达量的变化，探究这些Th/B细胞
免疫状态在CAN病程中的作用。方法：采用Fischer-Lewis 左肾原位移植法建立大鼠慢性移植性肾病模型，Lewis-Lewis 同种自体
移植作为对照组。所有受体大鼠，术后8 周处死，取脾脏组织，进行HE 染色，拍照后采用双盲法评价脾脏组织病理变化程度及淋
巴细胞浸润情况。用Trizol 法提取脾脏组织中总RNA，采用实时荧光定量聚合酶链式反应（qRT-PCR）法检测各组脾脏中Th1，
Th2，Th17，Treg 和B 细胞标志性因子的表达情况。结果：与对照组相比，CAN 大鼠脾脏出现明显的结构肿胀及淋巴细胞浸润增
多，并且Th1 细胞特征性因子IFN-γ和T-bet 表达量显著增加（P<0.001，P<0.05）；Th2 细胞特征性因子GATA-3 表达升高（P<0.
001），但IL-4无变化；IFN-γ/IL-4 比例明显上调（P<0.001），T-bet/GATA3 比例没有显著差异。Th17 的特征性因子IL-17 未见明显
改变，而Treg 细胞特征性因子Foxp3 表达增加（P<0.001），IL-17/Foxp3 平衡明显向Treg 细胞偏移（P<0.05）。B 细胞激活相关因子
TNFRSF13C 和RAG1表达量均显著上调（P<0.01，P<0.05），而RAG2 水平则没有变化。结论：CAN大鼠脾脏中Th1/Th2 的活性
平衡向Th1偏移，分化平衡未出现显著变化；Th17/Treg 的平衡向Treg 细胞偏移，B细胞免疫状态也被激活，这些变化在CAN病
程的发展中起到了重要作用，并且为临床监测和治疗提供了新的依据。 |
| 英文摘要: |
| Objective:To explore the immune statuses of splenic Th and B cells in a chronic allograft nephropathy (CAN) rat
model, and initially investigate the potential role of them in CAN.Methods:Chronic allograft nephropathy rat model was established by
orthotopic renal transplantation from Fischer to Lewis rats. Lewis to Lewis as the controls. Spleen samples were obtained 8 weeks after
transplantation. Sections of spleen tissues were stained by hematoxylin-eosin (HE), and the histopathology changes were elvaluated a
double-blind review. Besides, the expression of Th1/Th2/Th17/Treg/B-cell-associated immunological molecules were examined
byquantitative Real-time PCR (qRT-PCR).Results:There were splenic nodules swelling and a significant increase of lymphocyte
infiltration in the CAN group as compared to NC group. The expression of Th1-related molecules(IFN-γand T-bet) were increased (P<0.
001, P<0.05), while only Th2-related transcription factor GATA3 not IL-4 was enhanced (P<0.001). Similarly, IFN-γ/IL-4 rather than
T-bet/GATA3 was up-regulated in the spleen with CAN (P<0.001). Moreover, there were parallel Th17-related cytokines IL-17 level but
higher Foxp3 level of Treg (P<0.001), as well as lower IL-17/Foxp3 (P<0.05) in the CAN rats than NC individuals. Besides, the
expression of TNFRSF13C and RAG1 which involving in B cell activation were also improved in CAN recipients (P<0.01，P<0.05).Conclusion:The changes of these Th and B cell immune statuses in the spleen are closely associated with the pathological process of
CAN, which also provides a novel clue for monitoring this disease. |
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