| 李号令 季锋 周亚兰 周书转 吉栋 许华.IFN-γ参与右美托咪定在单关节炎大鼠模型中的镇痛作用[J].,2016,16(30):5847-5850 |
| IFN-γ参与右美托咪定在单关节炎大鼠模型中的镇痛作用 |
| Spinal IFN-γInvolves in Dexmedetomidine-induced Analgesia inMonoarthiritis Rats |
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| DOI： |
| 中文关键词: 右美托咪定 α2 肾上腺素受体 干扰素-γ 单关节炎 镇痛 |
| 英文关键词: Dexmedetomidine α2-adrenoceptor Interferon-gamma Monoarthritis Anti-nociceptive |
| 基金项目:国家自然科学基金项目(81070895) |
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| 中文摘要: |
| 目的：探讨IFN-γ是否参与了右美托咪定在大鼠单关节炎模型中的镇痛作用。方法：选用完全弗氏佐剂（CFA）诱导的大鼠单
关节炎模型，应用Von Frey纤毛检测大鼠造模以后以及造模并且鞘内连续给予右美托咪定后大鼠后足机械痛敏的变化。应用免
疫荧光组织化学实验检测大鼠脊髓背角IFN-γ的定位；应用蛋白质免疫印迹检测大鼠脊髓背角IFN-γ表达的变化。结果：大鼠单
关节炎模型建立后，脊髓背角IFN-γ的表达显著上调；大鼠脊髓IFN-酌定位于脊髓背角浅层；鞘内连续给予右美托咪定抑制了单
关节炎大鼠的机械痛敏，并且减少了单关节炎大鼠脊髓背角IFN-γ的表达。结论：脊髓背角IFN-酌的表达水平与右美托咪定对单
关节炎大鼠的镇痛作用有关。 |
| 英文摘要: |
| Objective:To investigate whether the analgesia effect of dexmedetomidine is associated with spinal IFN-γin
Monoarthiritis rats' model.Methods:The rat model of complete Freund's adjuvant (CFA)-induced ankle joint monoarthritis (MA) was
established. Von Frey hair was used to test the change of mechanical allodynia after CFA injection or CFA injection and
dexmedetomidine lumbar puncture (LP) injection. Immunohistochemistry and western blot was used to test the location and the
expression of IFN-γin the spinal dorsal horn.Results:Immunohistochemistry and western blot showed the increased IFN-γexpression in
the spinal dorsal horn. Immunofluorescence revealed that IFN-γwas localized to the superficial lamina of the spinal dorsal horn.
Inthrathecal administration of Dexmedetomidine inhibited the pain behavior and reduced IFN-γup-regulation in the ipsilateral spinal
dorsal horn.Conclusion:The spinal IFN-γis involved in the anti-nociceptive effect of dexmedetomidine in monoarthritis rats. |
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