欢迎访问《现代生物医学进展》编辑部！

目的：探讨腺病毒介导的转化生长因子β-1（TGF-β1）对结肠癌细胞凋亡的诱导作用。方法：结肠癌细胞系HCT116 细胞培养后分实验组及对照组，实验组以腺病毒为载体将TGF-β1 转染，逆转录聚合酶链反应（RT-PCR）以及免疫组织化学检测其mRNA 及蛋白的表达，MTT 法检测细胞生长抑制率，流式细胞仪检测细胞凋亡的情况。结果：实验组的TGF-β1 mRNA以及蛋白的表达明显增强；实验组细胞的吸光度波动较小，在低值区相对稳定，各时相点无明显变化，24 h 的细胞增殖抑制率为50%，其后在70% -80%之间；对照组细胞吸光度显著升高，与实验组各时相点比较，差异有统计学意义（P<0.01）。实验组24 h、36 h、48 h、60 h、72 h 的凋亡率分别为(7.55± 0.03) %、(8.53± 0.11) %、(13.47± 0.23) %、(15.51± 0.26) %、(16.59± 0.26) %，与对照组细胞各时相点比较差异有统计学意义（P<0.01）。结论：TGF-茁1 能够显著地抑制HCT 116 细胞的增值及诱导其凋亡。

英文摘要:

Objective:To investigate the inducing effect of adenovirus mediated transforming growth factor - beta1 (TGF-β1) on the cell apoptosis of colon cancer cells.Methods:HCT116 cells of human colon cancer were cultured and divided into the experimental group and the control group, cells in the experimental group were infected with recombinant adenovirus containing TGF-β1 in vitro, expression levels of TGF-β1 protein and mRNA were detected by immunohistochemical method and reverse transcription polymerase chain reaction (RT-PCR), the cell proliferation and apoptosis were detected by MTT method and flow cytometric.Results:The expression levels of TGF-β1 protein and mRNA in the experimental group was significantly increased; the absorbance of cells was smaller in the experimental group and stabled in low value area. The inhibition rate of proliferation was 50 %at 24 h and waved between 70%- 80 %in the following time; The absorbance of control group cells increased significantly compared with the cells in experimental group, and there were the significant difference statistically (P<0.01). The apoptosis rate in experimental group were (7.55± 0.03) %, (8.53± 0.11) %, (13.47± 0.23) %, (15.51± 0.26) %, (16.59± 0.26)%respectively at 24 h, 36 h, 48 h, 60 h and 72 h, The difference were significant statistically compared with the cells in experimental group (P<0.01).Conclusion:TGF-β1 could significantly inhibit the proliferation of HCT116 cells and induced its apoptosis.

查看全文查看/发表评论下载PDF阅读器

关闭