| 张欣 陈震霖 王洁 王菲 许欢.重楼皂苷VII对SW-480 细胞凋亡和周期的影响及机制研究[J].,2016,16(30):5809-5813 |
| 重楼皂苷VII对SW-480 细胞凋亡和周期的影响及机制研究 |
| Effects of Paris Saponin VII on the Apoptosis and the Cell Cycle of HumanColon Cancer SW-480 Cells and the Potential Mechanisms |
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| DOI： |
| 中文关键词: 重楼皂苷VII 结肠癌 细胞凋亡 细胞周期 |
| 英文关键词: Paris saponin VII Colon cancer Apoptosis Cell cycle |
| 基金项目:陕西省社会发展科技攻关项目（2015SF299） |
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| 中文摘要: |
| 目的：探讨重楼皂苷VII (Paris saponin VII, PSVII) 对人结肠癌SW480 细胞凋亡和周期的影响及其机制。方法：采用Cell
Counting Kit-8（CCk-8）试剂盒方法观察PSVII 对SW-480 细胞增殖的影响；流失细胞术研究PSVII对SW480细胞凋亡和周期的
作用；培养SW-480 细胞并给予1.0 umol/L、2.0 umol/L 和4.0 umol/L PSVII 24 h后，采用Western blot 法检测凋亡相关蛋白
Caspase-3、Caspase-8、Caspase-9、Bax和Bcl-2 及周期相关蛋白Cdk-4、Cdk-6、CyclinD1 和p21 的表达变化。结果：PSVII可明显抑
制SW-480 细胞增殖，并诱导其凋亡，其IC50值为5.25± 0.46 umol/L；PSVII 可促进SW-480 细胞Caspase-3、Caspase-8、Caspase-9、
Bax及p21 的表达，降低Bcl-2、Cdk-4、Cdk-6和CyclinD1 的表达。结论：PSVII可以通过线粒体和死亡受体途径诱导SW-480 细胞
凋亡；将SW-480 细胞周期阻滞于G1 期是通过上调p21，抑制Cdk-4、Cdk-6 与Cyclin D1 周期调控蛋白的表达。 |
| 英文摘要: |
| Objective:To investigate the effects of Paris saponin VII (PSVII) on the apoptosis and the cell cycle of human colon
cancer SW-480 cells and the potential mechanisms.Methods:The effects of different doses of PSVII on the proliferation of SW-480 cells
were observed by Cell Counting Kit-8（CCk-8）assay, and the effects of PSVII on the apoptosis and the cell cycle of SW-480 cells were
investigated by flow cytometric analysis. After treated with different concentration (1.0, 2.0, 4.0 umol/L) of PSVII , Western Blot was
used to test the expressions of Caspase-3, Caspase-8, Caspase-9, Bax and Bcl-2, and the cycle associated protein Cdk-4, Cdk-6, Cyclin
D1 and p21 in SW-480 cells.Results:PSVII could obviously inhibit the proliferation of SW-480 cells significantly in a dose dependent
manner. PSVII could induce the apoptosis of SW-480 cells and the IC50 was 5.25± 0.46 umol/L. PSVII could induce the apoptosis of
SW-480 cells by increasing the expression of Caspase-3, Caspase-8, Caspase-9 and Bax, while decreasing the expression of Bcl-2 in
SW-480 cells. PSVII could reduce the expression of Cdk-4, Cdk-6 and Cyclin D1, while increased the expression of p21 in SW-480 cells.Conclusion:PSVII can induce the apoptosis of SW-480 cells through both the death receptor pathway and mitochondrion pathway. PSVII
can arrest the cell Cyclein G1 phase by increasing the expression of p21, while decreasing the expression of Cdk-4, Cdk-6 and Cyclin D1
in SW-480 cells. |
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