| 董青川 程继 王志刚 刘全海 赵华才 赵文彩 张海燕 程永毅△.miR-155 通过p53/p21 调控前列腺癌细胞周期[J].,2016,16(29):5640-5643 |
| miR-155 通过p53/p21 调控前列腺癌细胞周期 |
| Effect of miR-155 on the Cell Cycle of Prostate Cancer by p53/p21Pathways |
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| DOI： |
| 中文关键词: miR-155 前列腺癌 细胞周期 p53 p21 |
| 英文关键词: miR-155 Prostate cancer Cell cycle p53 p21 |
| 基金项目:陕西省科技攻关项目(2010JM4043) |
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| 中文摘要: |
| 目的：探讨miR-155 对前列腺癌细胞周期的影响及其分子机制。方法：通过转染anti-miR-155 抑制前列腺癌DU145 和PC-3
细胞中miR-155 水平后，采用流式细胞术观察细胞周期的变化，western blot 和RT-PCR 观察p53 和p21蛋白及CDK2 和cyclin
蛋白和mRNA表达的变化。结果：与对照组相比，DU145 和PC-3 细胞转染anti-miR-155 后，G2/M期细胞阻滞，S期细胞数比例显
著增加(P<0.05)，p53 和p21 蛋白和mRNA表达水平显著增加(P<0.01)，CDK2 和cyclin E蛋白和mRNA 表达均显著降低(P<0.
01)。结论：miR-155 可影响人前列腺癌细胞的周期，可能与其调节p53、p21 及其下游的CDK2 和cyclin E的表达相关。 |
| 英文摘要: |
| Objective:To study the effects of miR-155 on the cell cycle of prostate cancer and the underlying mechanisms.Methods:The cell cycles of DU145 and PC-3 cells were evaluated after anti-miR-155 transfection by FACS. p53 and p21 expression were evaluated
by western blot and RT-PCR.Results:The cell cycle of DU145 and PC-3 cells were down regulated obviously after anti-miR-155
transfection(P<0.05), and the protein and mRNA expression of p53 and p21 were obviously increased (P<0.01). In addition, the protein
and mRNA expression of CDK2 and cyclin E were also decreased obviously (P<0.01).Conclusion:Inhibition of miR-155 could modulate
the cell cycles of DU145 and PC-3 cells in vitro by p53/p21 pathways and by down regulating the expression of CDK2 and cyclin E. |
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