| 桑玉兰 尹世琦 王冬杰 毕鑫 罗雯 霍建民 徐子平.COPD大鼠肺组织中小窝蛋白-1 的表达及其与气道重塑的关系[J].,2016,16(29):5634-5635 |
| COPD大鼠肺组织中小窝蛋白-1 的表达及其与气道重塑的关系 |
| Expression of Caveolin-1 in the Lung Tissues of Rats with COPD and ItsCorrelation with Airway Remodeling |
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| DOI： |
| 中文关键词: 小窝蛋白-1 气道重塑 慢性阻塞性肺疾病 炎性因子 |
| 英文关键词: Caveolin-1 Airway remodeling COPD Inflammatory factors |
| 基金项目:黑龙江省卫生厅科研课题（2012-552） |
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| 中文摘要: |
| 目的：探讨小窝蛋白-1(Caveolin-1)在COPD 大鼠肺组织中的表达及其与气道重塑的关系。方法：采用10 周龄Wister 大鼠
20 只，随机分为对照组与实验组，每组10 只，对照组常规饲养，实验组采用烟熏法+内毒素法建立COPD 模型。第91 天处死全部
大鼠，测定肺功能后取肺组织，采用免疫组织化学法检测Caveolin-1 的表达；酶联免疫吸附试验(ELISA)检测各组大鼠肺组织匀浆
中白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)的含量；分析Caveolin-1 的表达与IL-8、TNF-α、
TGF-β表达的相关性。结果：与对照组比较，实验组大鼠的肺顺应性明显下降，肺弹性阻力及气道阻力显著升高，肺组织TNF-α、
IL-8、TGF-β1 的含量明显升高，Caveolin-1 的表达明显降低，差异均有统计学意义(P<0.05)。大鼠肺组织中Caveolin-1的表达与
IL-8、TNF-α、TGF-β1 含量呈显著负相关(P<0.05)。结论：Caveolin-1 在COPD 大鼠肺组织中的表达明显降低，可能通过促进炎性细
胞因子的释放与气道重塑参与COPD 的发生和发展。 |
| 英文摘要: |
| Objective:To investigate the expression of caveolin-1(Caveolin-1) in the lung tissue of rats with COPD and its correlation
with airway remodeling.Methods:20 cases of 10 week-old Wister rats were randomly divided into the control group and the experimental
group, 10 rats in each group. The rats in control group were fed by normal feeding, while the rats in experimental group were fed
by the method of smoking and lipopolysaccharide (LPS). All the rats were killed at feeding for 91 days and the lung function were measured.
Then immunohistochemical method was used to detect the expression of caveolin-1 in lung tissue, and enzyme-linked immunosorbent
adsorption test (ELISA) was used to detect the interleukin IL-8 (IL-8), tumor necrosis factor alpha (TNF-α) and transformation
growth factor beta1 (TGF-β1) in lung tissue. The correlation of caveolin-1 expression with IL-8, TNF-αand TGF-β1 expression were
analyzed.Results:Compared with the control group, the lung adaptation of rats in experimental group decreased, lung elastic resistance
and airway resistance increased, the content of IL-8, TNF-αand TGF-β1 in lung tissue were significantly increased, the expression of
caveolin-1 in the lung tissue was significantly decreased (P<0.05). There was negative correlation of the caveolin-1 expression with IL-8,
TNF-α, TGF-β1 contents in the lung tissue.Conclusion:The expression of Caveolin-1 in the lung tissue of rats with COPD was significantly
decreased, Caveolin-1 might promote the release of inflammatory cytokines and airway remodeling, further participated in the development
of COPD. |
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