| 刘海涛 魏伟 唐翎.利用HPLC 法测定小牛血去蛋白滴眼液中尼泊金乙酯的含量[J].,2016,16(28):5428-5431 |
| 利用HPLC 法测定小牛血去蛋白滴眼液中尼泊金乙酯的含量 |
| Using HPLC Method to Quantify Ethylparaben in Deproteinized Calf Blood Extract Eye Drops |
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| DOI： |
| 中文关键词: 高效液相色谱法 小牛血去蛋白滴眼液 尼泊金乙酯 |
| 英文关键词: HPLC Deproteinized Calf Blood Extract Eye Drops Ethylparaben |
| 基金项目:湖南省科学技术厅科技计划项目（2013SK3014） |
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| 中文摘要: |
| 目的：探讨高效液相色谱法（HPLC）测定小牛血去蛋白滴眼液中尼泊金乙酯含量的可行性。方法：利用Agilent 1100 Series高
效液相色谱仪，在Diamonsil C18 色谱柱(250 mm × 4. 6 mm，5 um)，甲醇-0.05%乙酸水溶液（65:35）作为流动相，1. 0 mL·min -1流
速，和30 ℃柱温下，利用254 nm检测波长对小牛血去蛋白滴眼液中尼泊金乙酯进行定量分析。结果：尼泊金乙酯进样量在
0.29～1.43 ug 之间呈现良好的线性关系(r = 0.9999)，平均加样回收率为99.80%，RSD 为0.91%。结论：HPLC 方法简单快速，分离
效果好，可用于小牛血去蛋白滴眼液中尼泊金乙酯的定量测定。 |
| 英文摘要: |
| Objective:To develop an HPLC method to determine ethylparaben in deproteinized calf blood extract eye drops.Methods:The content of ethylparaben in deproteinized calf blood extract eye drops was measured and quantified by using Agilent 1100
Series HPLC with parameters as Diamonsil C18 column (250 mm× 4.6 mm, 5 um), 30℃ column temperature, methanol-0.05% acetic
acid at 1.0 mL·min-1 mobile phase, 10 滋L injection volume and 254 nm detection wavelength.Results:The method displayed good
linearity over the concentration ranges of 0.29～1.43 ug (r = 0.9999). The average recoveries of ethylparaben was 99.80%, with the RSD
of 0.91%.Conclusion:The HPLC method is suitable for the determination of ethylparaben in deproteinized calf blood extract eye drops
with its simple, accurate and reproducible features. |
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