| 王艳妨 包暄文 宋小杰 王冰 张英 孙广炜 马小军.中空硅纳米药物递送体系的线粒体靶向性探究[J].,2016,16(27):5201-5205 |
| 中空硅纳米药物递送体系的线粒体靶向性探究 |
| Hollow Silica Nanoparticles for Mitochondria-targeted Drug Delivery |
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| DOI： |
| 中文关键词: 中空硅纳米粒 线粒体靶向 药物递送 |
| 英文关键词: Hollow silica nanoparticles Mitochondria-targeted Drug-delivery system |
| 基金项目:国家自然科学基金项目（31470944; 21576254; 31271055） |
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| 中文摘要: |
| 目的：探究中空硅纳米粒在细胞线粒体内的定位。方法：以阿霉素为模型药物及荧光影像分子，通过激光共聚焦显微镜，考
察中空硅纳米粒与线粒体分子探针在线粒体内的共定位。通过差速离心法提取线粒体，进一步确认硅纳米粒在线粒体内的聚集。
结果：与线粒体分子探针为共定位参照，中孔硅纳米粒在线粒体中分布的皮尔逊系数为0.758± 0.033，线粒体提取结果显示，进入
细胞的纳米粒有90.01± 3.89%分布于线粒体。结论：中空硅纳米粒具有自发聚集于线粒体的能力，可以作为药物靶向递送载体，
为线粒体类疾病的治疗提供新的平台。 |
| 英文摘要: |
| Objective:To investigate the location of hollow silica nanoparticles in cells.Methods:Taken doxorubicin hydrochloride
as the modal drug and fluorescence imaging molecular, the location of silica nanoparticles in cells were co-located with Mito-tracker
Deep Red by laser scanning confocal microscope. The location of silica nanoparticles were further confirmed by isolation of mitochondrial
and cytosolic extracts through differential centrifugation.Results:Compared with Mitotracker Deep Red, the Pearson's Coefficient of the
silica nanoparticles was 0.758± 0.033, while the isolation of mitochondria showed 90.01± 3.89% of the model drug distributed in mitochondria.Conclusion:The hollow silica nanoparticles can spontaneously gathered in mitochondria, which provides a new platform to
mitochondria-targeted drug delivery systemfor mitochondria dysfunctional diseases. |
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