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目的：动态监测急性脑出血患者血清炎症因子和氧化应激产物水平，探讨其与患者病情及预后的关系。方法：采用双抗体夹心酶联免疫吸附法（ELISA 法）测定150 例急性脑出血患者（病例组）和120 例健康志愿者（对照组）发病24 h内、3 d、7 d及14 d 时血清炎症因子白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α），采用黄嘌呤氧化酶法（XTO）和硫代巴比妥酸法（TBA）测定两组的血清氧化应激产物超氧歧化酶（SOD）和丙二醛（MDA）水平，并分析血液炎症因子与氧化应激产物与患者病情及预后的关系。结果：病例组血清IL-6、TNF-α、SOD及MDA水平高于对照组，并在发病后7d 各指标水平达到最高，发病后14d 各指标水平低于发作≤ 24 h，差异有统计学意义（P<0.05）。大量出血组血清IL-6、TNF-α、SOD及MDA水平最高，中量出血组其次，小量出血组各指标水平最低，差异均有统计学意义（P<0.05）。重型组血清IL-6、TNF-α、SOD及MDA水平均最高，中型组其次，轻型组各指标水平最低，差异均有统计学意义（P<0.05）。结论：动态监测急性脑出血患者血清炎症因子及氧化应激产物水平有助于准确判断患者的病情及评估预后，临床有重要参考价值。

英文摘要:

Objective:To explore their relationship with the severity and prognosis of the patients by dynamic monitoring levels of seruminflammatory cytokines and products of oxidative stress in patients with acute cerebral hemorrhage(ACH).Methods:A total of 150 patients with ACH were chosen as case group and 120 healthy volunteers, as control group. The levels of seruminterleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) of the case group within 24 h, 3 d, 7 d, 14 d after admitted to the hospital and the control group were detected by double antibody sandwich enzyme linked immunosorbent assay(ELISA). The levels of superoxide dismutase(SOD) and malondialdehyde(MDA) of the two groups were detected by xanthine oxidase(XTO) method and thiobarbituric acid method(TBA). The relationship between serum inflammatory cytokines or products of oxidative stress and the condition and prognosis of the patients was analyzed.Results:The serum IL-6, TNF-alpha, SOD and MDA levels in the case group were higher than those in the control group.After 7 d, the level of each index in the case group all reached the highest and after 14 d, lower than these within 24 h, the difference was statistically significant (P<0.05). The levels of serum IL-6, TNF-alpha, SOD and MDA in massive hemorrhage group were the highest, these in moderate hemorrhage group, less and these in small hemorrhage group, the least; the differences were statistically significant (P<0.05).Conclusion:Dynamic monitoring of serum inflammatory cytokines and products of oxidative stress of the patients with acute cerebral hemorrhage is helpful in judging the condition and assess prognosis, which has an important reference value in the clinical application.

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