| 刘国丹 韩清△ 高翔春 王进 田苗.分子氢对糖尿病视网膜小胶质细胞的保护作用及其机制研究[J].,2016,16(26):5015-5018 |
| 分子氢对糖尿病视网膜小胶质细胞的保护作用及其机制研究 |
| A Study on the Protective Effect and Mechanismof Molecular Hydrogenon Diabetic Retinal Microglia Cells |
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| DOI： |
| 中文关键词: 糖尿病视网膜病变 氢 炎症 miRNA TLR4 |
| 英文关键词: Diabetic retinopathy Hydrogen Inflammation miRNA TLR4 |
| 基金项目:黑龙江省青年科学基金项目（QC2011C119） |
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| 中文摘要: |
| 目的：研究分子氢对糖尿病视网膜小胶质细胞的保护作用及其可能机制。方法：采用100 ng/mL的LPS诱导视网膜小胶质细
胞，同时将两组细胞分别置于正常培养环境和含有饱和氢培养环境下培养36 小时。RT-PCR 检测小胶质细胞中miR-9、miR-21 和
miR-199 的表达。Western Blot测定TLR4 信号途径相关蛋白的表达。结果：miR-9、miR-21 在分子氢作用后明显下调，而miR-199
在分子氢作用后下调不明显。并且，小胶质细胞活化后TLR4 途径相关信号蛋白的表达增加，在分子氢处理后Myd88 和IKKβ蛋
白的表达明显减少，而NF-κB蛋白的表达前后没有明显的变化。结论：分子氢对视网膜小胶质细胞的炎症损伤具有明显的保护作
用，氢作为一种信号分子对Myd88 介导的TLR4 炎症信号通路的调节及通路中部分miRNA 的调节作用可能是其抗炎作用的机
制。 |
| 英文摘要: |
| Objective:Molecular hydrogen was proved to be of neuroprotective effect to the retinal injury through its innate
regulatory mechanism and to provide the evidence that microRNAs are involved in the molecular pathogenesis of diabetic retinopathy.Methods:In present study, using Lipopolysaccharide (LPS)-activated retinal microglia model, we explored the potential mechanism of
molecular hydrogen in regulation of miRNA expression and signal-modulating activities. Retinal microglia was activated by LPS, and
then was treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression
difference of miR-9, miR-21 and miR-199 between these two groups.Results:The results demonstrated a dramatic down-regulation of
miR-9 and miR-21 by hydrogen treatment, while up-regulation of miR-199. Furthermore, we also detected the expression of LPS-induced
signaling proteins including Myd88, IKKβ, NF-κb, and PDCD4 by Western Blot. The data showed that the expression of Myd88 and
IKKβ were decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-κB
expression.Conclusion:The results in present study indicated that miR-9, miR-199 and miR-21 might play an important role in the
anti-inflammatory regulation of molecular hydrogen in LPS-activated microglias which will help further to explain the protective
mechanismof molecular hydrogen to inflammatory injury. |
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