| 王晓慧 崔忠涛 袁瑶薇 王香琛 刘宏.三氧化二砷对心肌细胞膜延迟整流钾通道蛋白表达的影响研究[J].,2016,16(24):4615-4617 |
| 三氧化二砷对心肌细胞膜延迟整流钾通道蛋白表达的影响研究 |
| Effects of Arsenic Trioxide on PotassiumChannel Protein Expression in the Hearts of Guinea Pig |
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| DOI： |
| 中文关键词: 三氧化二砷 QT 间期 KvLQT1 GERG |
| 英文关键词: As2O3 QTc KvLQT1 GERG |
| 基金项目:国家自然科学基金青年基金项目（81403288）；黑龙江省青年科学基金项目（QC2015102） |
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| 中文摘要: |
| 目的：观察不同剂量的三氧化二砷(arsenic trioxide，As2O3)对心肌细胞膜上延迟整流钾电流蛋白表达的影响。方法：将豚鼠随
机分为4 组：正常对照组、As2O3小剂量组(0.4 mg/kg)、中剂量组(0.8 mg/kg)、大剂量组(1.6 mg/kg)，给药后不同时间间隔记录心电
图，测量QT 间期和RR间期，计算QTc 的值的变化，同时应用荧光免疫组化技术检测心肌延迟整流钾通道IKr、IKs 通道蛋白的
表达量。结果：①在不同剂量的As2O3作用下，0.8 mg/kg和1.6 mg/kg As2O3组的豚鼠QTc 明显延长，并且这种延长作用与给药剂
量和时间密切相关。在2 h的观察时间内，0.8 mg/kg 和1.6 mg/kg As2O3分别使QTc 从对照组的324± 7 ms 延长到368± 11 ms
(P<0.01)和388± 11 ms(P<0.01)。②大剂量组豚鼠心肌缓慢型延迟整流钾通道KvLQT1 和GPERG 蛋白表达与对照组相比显著降
低(P<0.01)。结论：As2O3对豚鼠心肌QT 间期有明显延长效果，其机制可能与降低KvLQT1 和GPERG 蛋白的表达，影响了钾通道
的功能有关。 |
| 英文摘要: |
| Objective:To observe the effects of different doses of arsenic trioxide (trioxide arsenic, As2O3) on the expression of
delayed rectifier potassium current in cardiac muscle cells.Methods:Guinea pigs were divided into 4 groups randomly: control group,
low dose group (0.4 mg/kg), middle dose group (0.8 mg/kg) and high dose group (1.6 mg/kg). Electrocardiogram was recorded at
different time intervals after administration, QT interval and RR interval were measured to calculate the value of QTc. At the same time,
the expressionS of IKr and IKs channel protein in delayed rectifier potassium channel were detected by fluorescence
immunohistochemistry technique.Results:① In different doses of As2O3, 0.8 mg/kg and 1.6 As2O3mg/kg groups were significantly longer
in the QTc group, and the effect was closely related to the dosage and time. As2O3 at the doses of 0.8 mg/kg and 1.6 mg/kg, QTc was
gradually prolonged in the 2-hour period observation from 324± 7 ms of control to 368± 11 ms (P<0.01) and 388± 11 ms (P<0.01),
respectively. ②Compared with the control group, the expression of KvLQT1 and GPERG in the myocardium of the large dose group was
significantly lower than that of the control group (P<0.01).Conclusion:As2O3 had significant effect on the QT interval of guinea pig
myocardium, and its mechanismmight be related to the decrease of f KvLQT1 and GPERG protein expression, thus affecting the function
of potassiumchannel. |
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