| 谭超 赵晓光 袁建林 刘贺亮 韩超 张昆
张强 厐卫东 贺彦平 刘飞.BK通道对大鼠肾脏缺血再灌注损伤的作用及机制[J].,2016,16(23):4410-4414 |
| BK通道对大鼠肾脏缺血再灌注损伤的作用及机制 |
| The Role of BK Channel in Rat Renal Ischemia Reperfusion Injury andRelated Mechanisms |
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| DOI： |
| 中文关键词: 缺血再灌注 肾脏 大电导钙依赖性钾通道 α亚基 |
| 英文关键词: Ischemia Reperfusion Kidney Large conductance Ca2+-activated K+ channel αsubunit |
| 基金项目:国家自然科学基金项目（81270844） |
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| 中文摘要: |
| 目的：探讨缺血再灌注损伤早期肾脏皮质内大电导钙依赖性钾通道（BK）通道的表达及意义。方法：建立成年SD大鼠肾脏
急性缺血再灌注损伤模型，快速收集24 小时缺血大鼠与对照大鼠血和损伤侧肾脏皮质标本，使用ELISA 方法检测血肌酐和尿素
氮含量，实时荧光定量RT-PCR 和蛋白免疫印迹方法检测肾脏组织中BK通道α亚基的mRNA 表达水平和蛋白表达水平。结果：
（1）急性缺血再灌注大鼠损伤侧肾脏皮质BK通道α亚基mRNA水平较对照大鼠同侧肾脏皮质的表达明显降低（P< 0.01）。（2 ）急
性缺血再灌注大鼠损伤侧肾脏皮质BK通道α亚基蛋白水平较对照大鼠同侧肾脏皮质的表达也明显降低（P<0.05）。（3）NS1619
预处理缺血再灌注大鼠血尿素氮和血肌酐含量显著降低（P<0.05）。结论：BK通道表达和功能的改变是参与大鼠肾脏缺血再灌注
损伤的重要机制。 |
| 英文摘要: |
| Objective:To study the expression and effects of BK channel in the cortex of kidney after ischemia reperfusion injury.Methods:The model of Ischemia reperfusion in the kidney was developed in SD rats. The blood and the kidney tissues were collected at
24 h after IR. The values of BUM and Scr were tested by the ELISA, and the mRNA and protein expression of BK channelαsubunit
were examined by the RT-PCR and western blot.Results:The mRNA expression of BK channel αsubunit in IR group rats were were
significantly decreased compared with that in shamgroup rats(P<0.01). The protein expression level of BK channel αsubunit in IR group
rats were significantly lower than that in shamgroup rats(P<0.05). The values of BUMand Scr in IR rats after NS1619 treatment was significantly
decreased than the IR rats with saline.Conclusion:The expression and function of BK channel play an important rolein rat
renal ischemia reperfusion injury. |
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