| 杜小燕 覃华 田雯 张志武 侯颖.绞股蓝多糖对糖尿病肾病大鼠肾功能的保护作用及其机制研究[J].,2016,16(22):4247-4250 |
| 绞股蓝多糖对糖尿病肾病大鼠肾功能的保护作用及其机制研究 |
| Protection of Renal Function by the Polysaccharide fromGynostemmapentaphyllumon Diabetic Nephropathy and Its Mechanism |
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| DOI： |
| 中文关键词: 绞股蓝 多糖 糖尿病肾病 NF-kB |
| 英文关键词: Gynostemma pentaphyllum Polysaccharide Diabetic nephropathy NF-kB |
| 基金项目:陕西省社会发展科技攻关项目（2015SF196） |
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| 中文摘要: |
| 目的：探讨绞股蓝多糖对糖尿病大鼠肾功能的影响及其保护的可能机制。方法：采用大鼠高脂高糖饲料结合腹腔注射链脲佐
菌素建立2 型糖尿病模型。灌胃给予绞股蓝多糖8 周后，测定大鼠空腹血糖（FBG），血清肌酐、血清尿素氮、24 h尿量及尿蛋白等
指标；组织病理切片观察肾形态，肾小球体积；Western blot法检测肾皮质NF-κ B 的表达。结果：绞股蓝多糖（GPS）可剂量依赖性
的降低DN 组的FBG、血肌酐、24 h尿量和尿蛋白；但血尿素氮模型组与GPS 组无统计学差异；中、高剂量GPS 治疗后，肾脏指数
较模型组明显降低（P < 0.05）；与模型组（149.8 ± 12.2%）比较，高剂量GPS可抑制肾小球体积肥大至108.9 ± 9.6%。进一步研究
发现，GPS 可剂量依赖性的降低DN组肾脏NF-资B蛋白的表达，高、中剂量组水平与模型组相比有统计学差异（P < 0.05）。结论：
绞股蓝多糖对实验性糖尿病肾病具有保护作用，其机制可能与抑制肾炎症相关通路NF-kB 的表达有关。 |
| 英文摘要: |
| Objective:To investigate the protective effects of polysaccharide from Gynostemma pentaphyllum on type 2 diabetic
nephropathy and discuss the mechanisms.Methods:Diabetes rat model was induced by injection of streptozotocin after feeding high-fat
and high-sugar diet. The rats were divided into six groups. After the rats were treated with Gynostemma pentaphyllum polysaccharide or
positive control agent, enalapril for 8 weeks, the changes of fasting blood glucose (FBG), 24 h urine, urine protein and other biological
indexes were assessed. The pathological changes in the kidney were determined by H&E staining. The expression of NF-kB was detected
by western blotting.Results:Compared with model group, administration of the polysaccharide from Gynostemma pentaphyllum (GPS)
can reduce FBG, plasma creatinine, 24 h urinary volume and urine protein in a dose-dependent manner. But there was no significant
difference in blood urea nitrogen between model and GPS groups. After the 8-week treatment with medium or high dose of GPS, the
kidney index was significantly decreased. High dose of GPS can decrease the glomerulus volume to 108.9 ± 9.6%, compared to the
value of 149.8 ± 12.2% in model group. Furthermore,the expression of NF-kB protein in renal tissues was also significantly decreased.Conclusion:These data indicate that Gynostemma pentaphyllum polysaccharide protects renal function in rats of type 2 diabetes. The
mechanismmay be related with the inhibition of inflammation via NF-kB signaling pathway. |
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