| 修彬华 魏敏 邢国祥 马迎辉 闫志强.厄贝沙坦对脑损伤大鼠神经细胞凋亡的影响[J].,2016,16(22):4237-4241 |
| 厄贝沙坦对脑损伤大鼠神经细胞凋亡的影响 |
| The Anti-apoptosis Effects of Irbesartan on Fluid Percussion-induced BrainInjury in Rats* |
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| DOI： |
| 中文关键词: 单侧颅内动脉钝性损伤 厄贝沙坦 凋亡 AT1 受体 神经保护 |
| 英文关键词: Fluid percussion injury Irbesartan Apoptosis AT1 receptor Neuroprotection |
| 基金项目:全军医学科研课题面上项目（CWS11J235）；国家自然科学基金项目（81201000） |
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| 中文摘要: |
| 目的：研究血管紧张素I受体（AT1）抑制剂厄贝沙坦对侧位液压脑损伤模型大鼠神经细胞凋亡的影响。方法：利用改良的侧
位液压损伤装置建立大鼠颅脑损伤（TBI）模型，术前及术后给予厄贝沙坦治疗，用激光多普勒测定局部脑区血流（rCBF）的变化，
术前及术后1、3、5 和7d 利用神经功能评分评估大鼠神经功能损伤，利用TUNEL 染色检测大鼠脑细胞凋亡情况，利用Western
Blot检测大鼠脑组织损伤周围区域活性caspase 3的表达。结果：与正常值相比，TBI手术后损伤局部脑区rCBF下降至30 %（P<0.
05），神经功能评分显著降低(P<0.05)，损伤区周围脑组织TUNEL阳性细胞明显增多，活性caspase 3的表达显著增加(P<0.05)。厄
贝沙坦治疗组大鼠rCBF显著高于单纯TBI 组，梗死区面积显著缩小，神经功能得到明显改善，损伤区周围脑组织TUNEL 阳性
细胞和活性caspase 3表达下降(P 均<0.05)。结论：厄贝沙坦预处理能够通过抑制凋亡发挥神经保护作用。 |
| 英文摘要: |
| Objective:To investigate the neuroprotective effects of irbesartan on after fluid percussion-induced brain injury in rats.Methods:Traumatic brain injuries (TBI) model was prepared usingfluid percussion injury method and treated with irbesartan. Regional
cerebral blood flow (rCBF) and neurological status was evaluated at different time point respectively. Apoptosis was evaluated by
TdT-mediated dUTP nick end labeling (TUNEL) staining and active caspase 3 was detected.Results:Compared with sham group rats,
the rCBF was decreased to 30%in TBI rats (P<0.05). The neurological score was decreased significantly (P<0.05). TUNEL positive cells
and expression of active caspase 3 were increased in the peri-infarct cortex (P<0.05). Blockade of brain (angiotensin Ⅱ type 1)AT1
receptors with irbesartan before TBI could increase rCBF and reduce the infarct size on day 7 after TBI and increased neurologicalstatus
(P<0.05). TUNEL positive cells and expression of active caspase 3 were decreased in TBI rats after irbesartan treatment (P<0.05).Conclusion:Irbesartan can play neuroprotective effects by inhibiting apoptosis. |
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