| 高贺奇 曹璐 唐振宏 翟明明 刘娟 罗二平 景达.低强度振动载荷刺激对于三维多孔钛合金内成骨细胞粘附和增殖的影响[J].,2016,16(22):4233-4236 |
| 低强度振动载荷刺激对于三维多孔钛合金内成骨细胞粘附和增殖的影响 |
| Effect of Low-level Mechanical Vibration on Proliferation and Attachment ofOsteoblasts in 3-D Porous TitaniumImplants |
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| DOI： |
| 中文关键词: 低强度振动载荷 多孔钛合金 成骨细胞 细胞增殖 |
| 英文关键词: Low-level mechanical vibration Porous titaniumimplants Osteoblasts Cell proliferation |
| 基金项目:陕西省自然科学基金项目（2014JQ4139）；国家自然科学基金项目（31270889） |
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| 中文摘要: |
| 目的：探究低强度振动载荷刺激对于三维多孔钛合金（porous titanium，pTi）内兔成骨细胞（osteoblasts，OB）粘附和增殖的影
响，以期为临床振动载荷协同pTi治疗骨缺损提供重要的实验依据。方法：原代分离培养1 日龄新生新西兰兔颅盖骨OB，待传至
第3~6 代以5× 104/mL密度植入pTi中，随机分为振动组和对照组。振动组在5%CO2、37 ℃温度环境下进行3 天低强度振动载
荷（0.5 g，30 Hz）刺激，每天1 h；对照组放置在无载荷的振动平台上，分别采用DAPI 核染色法和MTT 法评价OB在pTi 中的粘
附和增殖情况。结果：在振动载荷刺激下，每个视野下OB 细胞在pTi 上的粘附数量14± 3个，对照组粘附数量6± 2 个，粘附能力
显著增强（P<0.05）；在振动载荷刺激下，pTi 上的OB 细胞增殖吸光度为36.5 %± 0.8 %，对照组吸光度为34 %± 1 %，细胞增殖能
力得到了显著促进（P<0.05）。结论：低强度振动载荷刺激对于提高pTi 中的OB 细胞生物学活性具有促进作用，本研究为下一步
系统探索振动载荷协同pTi对于骨缺损的修复效果奠定了基础。 |
| 英文摘要: |
| Objective:To determine the effect of low-level mechanical vibration on proliferation and attachment of osteoblasts in
the 3-D porous titaniumimplants (pTi), and thus provide critical experimental basis for combinative application of mechanical vibration
and pTi in the treatment of osseous defects in clinics.Methods:Primary osteoblasts(OB) were obtained by digesting the calvarial bone of
1-day-old New Zealand rabbits. Cells with passages 3~6 were used in the experiment. Primary osteoblasts were seeded onto the pTi
implants at a density of 5× 104 cells/mL, and randomLy divided into the control and mechanical vibration group. Cells in the mechanical
vibration group were subjected to 1 h/day mechanical vibratory stimulation (0.5 g, 30 Hz) for 3 days at room temperature. Cells in the
control group were placed onto the inactivated mechanical loading platform. MTT assays were performed to determine the cell
proliferation and DAPI assays were performed to evaluate the cell attachment in pTi.Results:The DAPI staining results demonstrate that
the osteoblastic attachment number in pTi in the mechanical vibration group was significantly higher than the control group (P<0.05).
MTT assays show that the mechanical vibration group showed increased cell proliferation as compared with the control group.Conclusion:Our present study shows that mechanical vibration improved the biological activities of osteoblasts in pTi, which lays the
foundation of the systemic investigation of pTi combined with mechanical vibration in bone defect repair. |
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