| 何峰 肖宗慧 刘卓 肖萍 姚海兰 冯淼 刘哲伟.紫外灭活CVB3 病毒诱导BALB/c小鼠产生免疫保护作用的研究[J].,2016,16(20):3801-3805 |
| 紫外灭活CVB3 病毒诱导BALB/c小鼠产生免疫保护作用的研究 |
| Study of Protective Immune Effect Induced by UV-inactivated CVB3 on the BALB/c Mice |
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| DOI： |
| 中文关键词: 紫外灭活型CVB3 细胞因子 中和抗体 免疫保护作用 |
| 英文关键词: UV-inactivated CVB3 Cytokines Neutralizing antibody Protective immune effect |
| 基金项目:国家自然科学基金项目（81271809）；北京市自然科学基金项目（7164241） |
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| 中文摘要: |
| 目的：探讨紫外灭活型CVB3 病毒诱导BALB/c 小鼠产生特异性免疫应答及保护作用的评估。方法：采用紫外灭活的方法处
理野生型CVB3 m株，按照0.1 LD50（104 PFU）的剂量免疫小鼠，设置PBS 免疫组作为对照，免疫后第3，5，7 天收集小鼠血清，检
测细胞因子含量；在第3，5，7，14 天分离小鼠脾脏，流式分析T 细胞亚群的分布比例；在免疫后一个月分离小鼠血清，检测中和抗
体的滴度；同时间给予小鼠100LD50 野生型CVB3 感染，观察小鼠的死亡率。结果：与对照组相比，在检测日期内紫外灭活型
CVB3 组小鼠血清中细胞因子IL-1alpha，TNF-alpha，IL-6 的表达量明显增高（P<0.05），IL-4 的表达量没有明显差异；免疫后第14 天
CD3+CD4+T细胞的分布较对照组明显升高（P<0.05）；在免疫后一个月，紫外灭活型CVB3 免疫组可以诱导机体产生高滴度中和
抗体，同时，小鼠应对高致死量CVB3 感染时有较高的存活率。结论：紫外灭活型CVB3 感染能诱导机体产生特异性免疫应答，同
时，产生的中和抗体可以提高小鼠应对致死剂量CVB3 感染时的生存率，对机体有明显的保护作用。 |
| 英文摘要: |
| Objective:To investigate specific immune response and protective effect of BALB/c mice induced by UV inactivated
CVB3.Methods:Wild CVB3 mstrain was inactivated by UV, and male BALB/c mice were immunized with 0.1 LD50 (104 PFU) of UV
inactivated CVB3, named CVB3-UV, PBS treated mice were set as compared group. On the 3, 5, 7 days post immunization mice were
sacrificed, serum were collected and cytokines were detected by CBA method, T cell subset were analyzed by flow cytometry method in
the splenocytes on the 3, 5, 7, 14 days post immunization, neutralizing antibody in the sera of immunized mice 1 month later were
detected, further, mice survival rate were also evaluated after challenge with lethal dose of wild type CVB3.Results:Compared with PBS
treated group, CVB3-UV could induce high levels of IL-1alpha, TNF-alpha and IL-6 (P<0.05), while IL-4 production was comparable between
both groups. CD3+CD4+T cell development were found affected on the 14 days post immunization by CVB3-UV (P<0.05), and higher
titer neutralizing antibody were induced in the CVB3-UV treated mice. Importantly, higher survival rate of mice were found in the
CVB3-UV group after re-infection by lethal CVB3.Conclusion:UV-inactivated CVB3 could induce specific immune response and
support obvious protective effect in BALB/c mice. |
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