| 郑宸 应长青 洪心 卢婷婷 李旭颖 尹凡硕.嗜热菌Anoxybacillu sp.产淀粉酶培养基优化及产酶条件研究[J].,2016,16(19):3619-3624 |
| 嗜热菌Anoxybacillu sp.产淀粉酶培养基优化及产酶条件研究 |
| Optimization of Producing Amylase Mediumand Production Condition ofThermophiles Anoxybacillu sp. |
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| DOI： |
| 中文关键词: 嗜热菌 淀粉酶 培养基优化 产酶条件 |
| 英文关键词: Thermophilic bacteria Amylase Culture mediumoptimization Production conditions |
| 基金项目:黑龙江省教育厅科技项目(12521217) |
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| 中文摘要: |
| 目的：对产淀粉酶嗜热菌Anoxybacillu sp.菌株进行培养基优化及产酶条件研究，以便提高菌株的产酶能力，并为下一步菌
株的诱变育种研究提供基础。方法：常规方法液体培养菌株，用平板初筛和DNS法复筛选择产淀粉酶能力较高的菌株；单因素筛
选培养基最适的碳源、氮源、Ca2+浓度和Mg2+浓度，对单因素筛选的最佳碳源、氮源、Ca2+和Mg2+的三个较佳浓度进行四因素三
水平正交试验优化培养基；对培养基不同pH值及不同培养温度进行培养条件研究。结果：产淀粉酶菌株筛选结果显示：六株菌中
淀粉酶酶活力值最大的是菌株Anoxybacillu sp.DL4，差异有统计学意义（P＜0.05）。培养基单因素筛选结果显示：最适碳源为麦芽糖、最适氮源为
硝酸铵、最适Ca2+、Mg2+浓度均为0.02%，差异有统计学意义（P＜0.05）。培养基优化结果显示：C 源0.1 %，N源0.2 %，Mg2+ 0.04%，
Ca2+ 0.04 %为最佳的培养基成分组合。产酶条件筛选结果显示：培养基pH 值为6、培养温度为55 ℃时菌株产酶水平最高，差异有
统计学意义（P＜0.05）。结论：培养基的优化及最适的产酶条件能提高嗜热菌Anoxybacillu sp.DL4 产淀粉酶能力，Ca2+、Mg2+离子
对菌株产淀粉酶有促进作用。 |
| 英文摘要: |
| Objective:To improve the enzyme-producing capability and provide a basis for further study on mutation breeding of
strain, we studied optimization of the culture medium and conditions of the enzyme production of thermophilic bacteria Anoxybacillu sp.Methods:Conventional method was used to culture the strains. The high-yield amylase strains were finally obtained through the first
screening with tablet screening with and the second screening with DNS method. The single-factor screening was used to screen the optimal
carbon source, nitrogen source, Ca2+ concentrations and Mg2+ concentrations in the culture medium. The optimal composition based
on the single-factor screening was optimized by orthogonal test of four factors and three levels. Single-factor screening was also used for
selection of the pH of medium, the optimum culture temperature.Results:The results of screening for the amylase-producing strains
showed that the strain DL4 has the highest amylase activity among the six strains (P＜0.05). The univariate screening results showed that
maltose was the optimumcarbon source (P＜0.05), ammoniumnitrate was the best carbon source (P＜0.05), and the optimal concentrations
of Ca2+ and Mg2+ were all 0.02%(P＜0.05). Mediumoptimization results showed that C source of 0.1 %, N source 0.2 %, Mg2+ 0.04
%, and Ca2+ 0.04 % were the best culture medium compositions. The result of screening for conditions of enzyme production indicated
that Anoxybacillu sp . DL4 produced the highest enzyme levels at pH 6 (P＜0.05) for 55 ℃ (P＜0.05).Conclusion:The optimization of
the culture medium and the optimum conditions of enzyme production could improve the amylase production capacity of
Anoxybacillu sp.DL4, and Ca2+ and Mg2+ could contribute to the strains of the enzyme-producing ability. |
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