刘强 苏玉 裴国田 王帅 杨影顺 闵先军 黄宇清 王维.基于RNA干扰CMTM7 对肺腺癌A549细胞凋亡的影响[J].,2016,16(18):3431-3434 |
基于RNA干扰CMTM7 对肺腺癌A549细胞凋亡的影响 |
Effects of RNA Interference CMTM7 on Apoptosis of Lung AdenocarcinomaA549 Cells |
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DOI: |
中文关键词: CMTM7 RNA 干扰 细胞凋亡 肺腺癌A549 细胞 细胞周期 |
英文关键词: CMTM7 RNA interference Apoptosis Lung adenocarcinoma A549 Cell cycle |
基金项目:河北省卫生厅2013 年医学科学研究项目(20130446);
海淀区属卫生系统高层次专业技术人才队伍建设人才培养专项资金项目[海公共委(2014)] |
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中文摘要: |
目的:探讨基于RNA 干扰CMTM7 对肺腺癌A549细胞凋亡的影响。方法:选择肺腺癌A549 细胞株分为siRNA 组、阴性对
照组与空白对照组,在对数生长的A549 细胞中转染RNA 干扰CMTM7 载体、脂质体空载体和不进行转染,观察A549 细胞的生
长、凋亡与细胞周期状况。结果:MTT 实验显示siRNA转染组的抑制率明显高于阴性对照组和空白对照组(P<0.05),阴性对照组
与空白对照组的对比无明显差异(P>0.05)。流式细胞术实验表明siRNA 转染组的细胞凋亡率明显高于阴性对照组和空白对照组
(P<0.05),阴性对照组与空白对照组的对比无明显差异(P>0.05)。流式细胞术实验表明siRNA转染组的G0/G1 期细胞数目较阴性
对照组和空白对照组增多明显(P<0.05),同时siRNA转染组的S、G2/M 期细胞数目较阴性对照组和空白对照组明显减少(P<0.
05),阴性对照组和空白对照组对比差异无统计学意义(P>0.05)。结论:RNA 干扰CMTM7 能够促进肺腺癌A549细胞凋亡,抑制肿
瘤细胞的生长,其作用机制可能通过干扰细胞周期而实现。 |
英文摘要: |
Objective:To investigate the effects of RNA interference CMTM7 on apoptosis of lung adenocarcinoma A549 cell by
the.Methods:Selected lung adenocarcinoma A549 cell were divided into siRNA group, negative control group and blank control group,
and the RNA interference CMTM7 vectors were transfected into the logarithmic growth of A549 cells, observed A549 cell growth, and
the apoptosis and cell cycle status was detected.Results:MTT experiment showed that the inhibition rate in siRNA transfection group
was significantly higher than that of the negative control group and blank control group (P <0.05), while the difference between the
negative control group and the control group were not significant (P> 0.05). Flow cytometry showed the apoptosis rate in the siRNA
transfection group was significantly higher than that of the negative control group and blank control group (P <0.05), while the difference
between the negative control group and the control group were not significant (P> 0.05). Flow cytometry experiments showed that the
number of G0 / G1 phase cell in the siRNA transfection group was increased with significantly difference compared with blank control
group and negative control group (P <0.05), while the number of G2 /Mphase cell in the siRNA transfection group was less than that of
the control group and negative control group (P <0.05), but it had no significant difference between the blank control group and negative
control group (P> 0.05).Conclusion:RNA interference CMTM7 can promote lung adenocarcinoma A549 cell apoptosis, inhibit the
growth of tumor cells, and its mechanismmay be achieved by interfering with the cell cycle. |
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