| 师惠 巩思嘉 赖姨梅 程丽宪 李晓曦 林艳 赵子建△.脂联素在应激状态下对小鼠卵泡发育的影响[J].,2016,16(18):3405-3408 |
| 脂联素在应激状态下对小鼠卵泡发育的影响 |
| Effect of Adiponectin on Follicular Development Under Stress in Mice |
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| DOI： |
| 中文关键词: 饥饿 脂联素 卵泡发育 |
| 英文关键词: Starvation Adiponectin Follicle development |
| 基金项目:国家重大科学研究计划（2013CB945200）；江苏省自然科学基金项目（BK20130059，BK2011766）；
国家自然科学基金项目（81372798，81200570）；江苏省高校自然科学研究计划项目（12KJB310006） |
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| 中文摘要: |
| 目的：利用饥饿刺激探讨应激状态下脂联素缺失对小鼠卵泡发育的影响。方法：C57BL/6、脂联素半缺失（APN+/-）、脂联素全
缺失(APN-/-)三种基因型小鼠以正常进食量的一半给予饥饿刺激，建立应激模型，30 天后处死小鼠取卵巢，计数三种基因型小鼠
各级卵泡数目。结果：①经过30 天饥饿后饥饿组小鼠体重下降与初始体重比较有统计学意义(P<0.001)，应激模型建立。②应激状
态下三种基因型小鼠卵巢重量未见统计学差异(P>0.05)。③正常饮食组三种基因型小鼠各级卵泡比例未有统计学差异(P>0.05)。
饥饿后C57BL/6 小鼠原始卵泡比例为(47± 2.966)%，APN+/- 原始卵泡比例为(36.5± 1.555)%（P<0.05），APN-/-原始卵泡比例为
(36.8± 2.200)%(P<0.05)；C57BL/6 闭锁卵泡比例为（12± 1.225）%，APN+/-闭锁卵泡比例为（19.75± 1.887）%(P<0.01)，APN-/-闭锁
卵泡比例为（20± 0.8367）%(P<0.001)，与野生型闭锁卵泡比例比较差异有统计学意义。结论：饥饿刺激下脂联素缺失小鼠原始卵
泡消耗增加，而闭锁卵泡的发生增加，处于生长状态的卵泡减少，提示在应激状态下脂联素水平的下降可导致卵泡成熟的过程受
阻。 |
| 英文摘要: |
| Objective:To study the effect of adiponectin on the follicular development in mice under stress.Methods:C57B6/L,
Adiponectin heterozygotic (APN+/-), Adiponectin homozygotic (APN-/-) were fed by half of the diet. The mice were killed after 30 days,
and the number of follicles was counted and analyzed.Results:① After 30 days of starvation, the weight of the mice was decreased
significantly (P<0.001), and the model of stress were established. ② The weight of ovary showed no difference between the three
genetypes under stress (P>0.05). ③ The three genotypes of normal diet showed no significant difference in the percentage of follicles
(P>0.05). The percentage of primordial follicles in the wild type mice, heterozygotic and homozygotic mice were (47± 2.966)%, (36.5±
1.555)%and (36.8± 2.200)%, respectively, and the difference had statistical significance (P<0.05). The percentages of atretic follicles in
the wild type was (12 ± 1.225)%, while the percentages of atretic follicles was (19.75 ± 1.887)% (P<0.01) in the adiponectin
heterozygotic mice and (20.00± 0.8367)%(P<0.001) in the adiponectin KO mice , which was statistically significant compared with that
of the wild type mice.Conclusion:Starvation can stimulate the consumption of the primordial follicles of adiponectin deficient mice,
while the number of atretic follicles is increased, suggesting that the decrease of adiponectin levels under stress can lead to the obstruction
of follicular maturation process. |
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