| 孙龙和 夏建国 钱春华 汤民 钱明兰.白藜芦醇通过Pi3K/AKT 通路抑制胃癌SGC-7901 细胞增殖和迁移[J].,2016,16(17):3637-3240 |
| 白藜芦醇通过Pi3K/AKT 通路抑制胃癌SGC-7901 细胞增殖和迁移 |
| Resveratrol Inhibit Gastric Carcinoma Cell SGC-7901 Proliferation andMigration by Inhibiting Pi3K/AKT Pathway |
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| DOI： |
| 中文关键词: 胃癌SGC-7901 细胞 白藜芦醇 Pi3K/AKT 增殖 迁移 |
| 英文关键词: SGC-7901 cell Resveratrol Pi3K/AKT Proliferation Migration |
| 基金项目: |
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| 中文摘要: |
| 目的：验证白藜芦醇是否可以抑制胃癌SGC-7901 细胞增殖和迁移及其信号通路。方法：用不同浓度白藜芦醇干预
SGC-7901 细胞，再用LY-294002 和IGF-1 分别用来抑制和激活Pi3K/AKT 通路。MTT 法测细胞增殖，划痕试验和Transwell 试验
测细胞迁移，Western blot 检测细胞迁移相关蛋白（MMP-2、MMP-9）、细胞迁移相关蛋白（P21、P27）、以及AKT、p-AKT 的表达情
况；结果：相比于对照组，白藜芦醇组胃癌细胞增殖和迁移减弱(P=0.001)，p-AKT表达减少(P<0.001)；LY-294002 可以抑制p-AKT
的表达(P=0.004)，和白藜芦醇一样可以抑制胃癌细胞的增殖和迁移；IGF-1 可以显著增加p-AKT的表达(P<0.001)，可以逆转白藜
芦醇对胃癌细胞增殖和迁移的抑制作用。结论：白藜芦醇通过抑制Pi3K/AKT 信号通路抑制胃癌细胞增殖和迁移。 |
| 英文摘要: |
| Objective:To verify whether resveratrol could inhibit SGC-7901 cells proliferation and migration through Pi3K/AKT
pathway.Methods:Different concentrations of resveratrol were used to incubate with SGC-79011 cells. LY-294002 and IGF-1 were used
to inhibit and activate Pi3K/AKT pathway respectively. MTT were used to investigate the proliferation of SGC-7901 cells. Transwell
chambers and wound healing were employed to test the migratory ability of SGC-79011 cells. Western blotting were used to investigate
the expressions of P21, P27, MMP-2, MMP-9, AKT and p-AKT inSGC-7901 cells.Results:Compared with the control group, the proliferation
and migration of SGC-7901 cells and the expression of p-AKT were decreased in the resveratrol group (P=0.001); the inhibitory
effect of the downregulation of p-AKT by LY-294002on the induction of SGC-7901 cells proliferation and migration was identical to that
of resveratro (P<0.001); upregulation of p-AKT by IGF-1reversed the resveratrol-induced inhibition of SGC-7901 cells dedifferentiation
(P<0.001).Conclusion:Resveratrol inhibits SGC-7901 cells proliferation and migration by suppressing Pi3K/AKT signaling pathway. |
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