| 郝广华 汪南平 黄雅姿 赵蓓蕾 张弘.视黄醇结合蛋白4 对血管平滑肌细胞迁移和增殖的影响及机制[J].,2016,16(17):3212-3216 |
| 视黄醇结合蛋白4 对血管平滑肌细胞迁移和增殖的影响及机制 |
| Role of RBP4 in Vascular Smooth Muscle Cells Migration and Proliferationand Its Molecular Mechanism |
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| DOI： |
| 中文关键词: 视黄醇结合蛋白 动脉粥样硬化 平滑肌细胞 迁移 增殖 |
| 英文关键词: Retinol binding protein Atherosclerosis Smooth muscle cell Migration Proliferation |
| 基金项目:国家自然科学基金项目（30900693）；中国博士后科学基金会基金项目(20080440284) |
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| 中文摘要: |
| 目的：研究视黄醇结合蛋白4（Retinol-binding protein 4，RBP4）对血管平滑肌细胞（SMCs）迁移和增殖的影响及分子机制。方
法：体外培养大鼠主动脉SMCs，采用划痕实验及Boyden's 迁移小室实验观察RBP4 对SMCs 迁移的影响，采用免疫印迹实验技
术检测Akt 的磷酸化水平，采用Boyden's 小室实验观察PI3K 抑制剂LY294002 预处理细胞对RBP4 促SMCs 迁移的影响，应用
MTT 比色实验结合流式细胞仪技术，检测RBP4 对SMCs 细胞增殖及细胞周期的影响。结果：RBP4 呈剂量依赖性诱导大鼠血管
SMCs迁移（P<0.05）；RBP4 处理细胞显著增加了Akt 磷酸化；PI3K 抑制剂LY294002 预处理细胞则显著抑制了RBP4的促迁移
作用（P<0.05）；RBP4 处理有增加SMCs数量的趋势，且可轻微阻滞细胞进入S期，但未达到统计学显著性（P>0.05）。结论：RBP4
通过PI3K-Akt通路诱导大鼠血管SMCs迁移，对细胞增殖及细胞周期则无显著影响。 |
| 英文摘要: |
| Objective:To explore a potential role of retinol-binding protein 4 (RBP4) in vascular smooth muscle cells (SMCs) migration
and proliferation and its molecular mechanism.Methods:Vascular SMCs of rat were cultured in vitro. Vascular SMCs migration
was studied by scratch assay and Boyden's chamber. Western blot analysis was used for detecting Akt and phospho-Akt protein levels.
SMCs were pretreated with PI3K inhibitor LY294002, Boyden's chamber was then used for detecting RBP4-induced cell migration. Cell
proliferation and cell cycle of vascular SMCs induced by RBP4 was detected by use of MTT dye absorbance and flow cytometry.Results:Recombinant human RBP4 significantly induced migration of rat vascular SMCs in a dose-dependent fashion (P<0.05) and phosphorylation
of Akt at the same time. PI3K inhibitor Ly294002 significantly suppressed RBP4's effects on cell migration (P<0.05). RBP4 treatment
slightly increased SMCs proliferation and led to G1/S cell cycle arrest, but these effects did not reach statistical significance.Conclusion:RBP4 induces migration of vascular SMCs through PI3K-Akt-dependent pathways. However, the proliferation and cell cycle
progression of the SMCs is not affected by RBP4 treatment. |
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