| 刘媛媛 康凯 杨威 彭相虹 杨松柳 赵鸣雁△.银杏内酯B 对星形胶质细胞氧化损伤的保护作用[J].,2016,16(16):3010-3013 |
| 银杏内酯B 对星形胶质细胞氧化损伤的保护作用 |
| Protective Effect of Ginkgolide B on Astrocytes Oxidative Damage fromOxidative Damage |
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| DOI： |
| 中文关键词: 银杏内酯B H2O2 星形胶质细胞 氧化损伤 |
| 英文关键词: Ginkgolide B H2O2 Astrocytes Oxidative damage |
| 基金项目:国家自然科学基金项目（81171785） |
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| 中文摘要: |
| 目的：探讨银杏内酯B对过氧化氢(H2O2) 诱导的星形胶质细胞损伤的保护作用及可能机制。方法：星形胶质细胞传代培养，
分为阴性对照组(以正常培养液培养)，氧化损伤组(100 滋mol·L-1的H2O2作用12 h)，银杏内酯B 低剂量组(1× 10-6mol·L-1银杏内
酯B孵育24 h后，加入H2O2作用12 h)和银杏内酯B 高剂量组(1× 10-4mol·L-1银杏内酯B 孵育24 h后，加入H2O2作用12 h)，
MTT 比色法检测细胞存活率，流式细胞仪检测细胞活性氧(ROS)水平，分光光度计检测上清液中超氧化物歧化酶(SOD)、如谷胱
甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)的含量。结果：银杏内酯B能抑制氧化损伤引起的细胞活性的下降，降低星形胶质
细胞内ROS的生成，促进SOD、GSH-Px 水平的升高及MDA 水平的下降。结论：银杏内酯B 通过提高细胞内SOD、GSH-Px 含
量，降低细胞内MDA含量发挥其较强的抗氧化作用，从而为其用于治疗神经系统疾病提供可靠的实验依据。 |
| 英文摘要: |
| Objective:To investigate the protective effect and possible mechanisms of ginkgolide B on astrocytes induced by oxidative
damage.Methods:Astrocytes were subcultured and divided into negative control group (cultured with normal medium), oxidative
injury group (100 umol·L-1 H2O2 treated for 12 h), ginkgolide B low dose group (1× 10-6 mol·L-1 ginkgolide B treated for 24 h, then 100
滋mol·L-1 H2O2 treated for 12 h), ginkgolide B high dose group (1× 10-4 mol·L-1 ginkgolide B treated for 24 h, then 100 umol·L-1 H2O2
treated for 12 h), cell viability was assayed by MTT colorimetric, reactive oxygen species (ROS) levels were detected by flow cytometry,
content of superoxide dismutase (SOD) glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in supernatants were detected by
spectrophotometer.Results:Ginkgolide B could inhibit oxidative damage induced cell activity decline, decreased the generation of ROS
in astrocytes, promote the rise of levels of SOD, GSH-Px and decline of MDA.Conclusion:Ginkgolide B play its strong antioxidant effect
by increasing the intracellular content of SOD, GSH-Px levels and decreasing MDA levels, which provide reliable experimental basis
for treatment of cataracts. |
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