| 丛薇 余琛琳 林丽芳 赵善民 杨文静 蔡丽萍 汤球 孙伟 徐晨 李莉△.黄曲霉素对裸鼹鼠肺泡上皮Ⅱ型细胞基因表达的影响[J].,2016,16(15):2847-2851 |
| 黄曲霉素对裸鼹鼠肺泡上皮Ⅱ型细胞基因表达的影响 |
| Effects of Aflatoxin on the Gene-expression in Type II Alveolar-epithelialCells of Heterocephalus Glaber |
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| DOI： |
| 中文关键词: 肺泡上皮Ⅱ型细胞 黄曲霉素 裸鼹鼠 炎症因子 |
| 英文关键词: AEC II Aflatoxin Heterocephalus glaber Inflammation factor |
| 基金项目:国家科技支撑计划（2015BAI09B02）；上海市科委基金项目（14140900200；15140900200） |
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| 中文摘要: |
| 目的：观察不同浓度黄曲霉素对裸鼹鼠肺泡上皮Ⅱ型细胞(AEC II)相关因子mRNA表达的影响，探讨裸鼹鼠抵御肺癌的分
子机制。方法：通过酶消化法分离裸鼹鼠肺组织细胞并用免疫黏附法进行纯化得到裸鼹鼠肺泡上皮Ⅱ型细胞进行原代培养，40 小
时后，实验组分别给予不同浓度(0.25、0.5、1.0、2.0 和4.0 mg/L)的黄曲霉素处理，溶剂对照组给予DMSO(0.4 mL/L)处理。黄曲霉素
作用48 小时后收集细胞，用实时定量PCR 技术检测裸鼹鼠AECII 细胞中的SP-C 基因及TNF-琢、IL-1、IL-6、IL-8、IL-12 等炎症因
子的mRNA 表达变化。结果：原代分离的裸鼹鼠AEC II细胞纯度＞70 %，活性＞90 %，可用于体外实验。定量PCR 结果显示黄曲
霉素使裸鼹鼠AEC II 细胞SP-C 表达水平显著下降，TNF-琢、IL-1、IL-6、IL-8、IL-12 等炎症因子的表达水平在黄曲霉低于2 mg/L
浓度的条件下没有显著变化。结论：裸鼹鼠AEC II细胞在黄曲霉素导致细胞受损的情况下，仍然保持较低水平的炎症因子表达，
这可能是其抵抗肺癌的机制之一。 |
| 英文摘要: |
| Objective:To investigate the effects on Heterocephalus glaber alveolar epithelial type II cells (AEC II) related factors
mRNA expression at different concentration of aflatoxin (AF) and explore the molecular mechanism of Heterocephalus glaber against
lung cancer.Methods:The lung tissue of Heterocephalus glaber was digested with enzymes and purified by immune adherence for primary
culture. After being cultured for 40 h, the primary cultured cells were randomly divided into 6 groups: AF were added to the medium at
final concentrations of 0.25, 0.5, 1.0, 2.0 and 4.0 mg/L as AF treated groups, DMSO (0.4 mL/L) was added to the mediumof solvent control
group. The cells were incubated with AF or DMSO for 48 h, and harvested. The expression levels of SP-C, TNF-alpha, IL-1, IL-6, IL-8
and IL-12 in AEC II cells were detected with real-time quantitative PCR.Results:AEC II cells of Heterocephalus glaber were harvested
with a purity of above 70 %and a viability of above 90 %, which meeted the requirement in vitro experiments. Quantitative PCR results
showed AF made the expression levels of SP-C in Heterocephalus glaber AEC II cells significantly decreased. In the low concentrations
of AF groups, the expression levels of TNF-alpha, IL-1, IL-6, IL-8 and IL-12 in Heterocephalus glaber AEC II cells showed no statistically
significant difference as control.Conclusion:In the case of cell damage caused by AF, Heterocephalus glaber AEC II cells remained relatively
low levels of inflammation factor expression. This might be one of the resistance mechanismagainst lung cancer. |
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