| 宋苗苗 雷珂 董增义 陈志红.Toll 样受体4在小于胎龄儿生后发生胰岛素抵抗的作用研究[J].,2016,16(14):2631-2636 |
| Toll 样受体4在小于胎龄儿生后发生胰岛素抵抗的作用研究 |
| The Effect of the Tool-like Receptor 4 Signaling Pathway on InsulinResistance in Small for Gestational Age Rats |
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| DOI： |
| 中文关键词: Toll样受体4 小于胎龄儿 追赶生长 胰岛素抵抗 |
| 英文关键词: Toll-like receptor 4 Small for gestational age Catch-up growth Insulin resistance |
| 基金项目:青岛市科技计划项目（13-1-3-28-nsh）；国家自然科学基金项目（81170762） |
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| 中文摘要: |
| 目的：探讨Toll 样受体4 在小于胎龄儿生后发生胰岛素抵抗的作用。方法：建立动物模型，分为小于胎龄儿追赶生长组(S1
组)、小于胎龄儿无追赶生长组（S2 组）、适于胎龄儿组（AGA组）。生后4 周和12 周取血、肝脏和脂肪组织，检测血糖、胰岛素、甘
油三酯、游离脂肪酸和总胆固醇，计算胰岛素抵抗指数（Homeostasis model assessment for insulin resistance index, HOMA-IR)；
ELISA法检测血清白介素-6、肿瘤坏死因子-alpha；实时定量RT-PCR法检测相同体质量肝脏和脂肪组织中Toll 样受体4、髓细胞样
分化因子88、核因子kB、肿瘤坏死因子-alpha和白介素-6 mRNA 的表达。结果：与适于胎龄儿组和小于胎龄儿无追赶生长组相比，
小于胎龄儿追赶生长组随年龄增长血糖、血清胰岛素、游离脂肪酸、甘油三酯和HOMA-IR 逐渐增高（P＜0.05），相同体质量肝脏
和脂肪组织中Toll 样受体4、髓细胞样分化因子88、核因子资B、肿瘤坏死因子-琢和白介素-6 mRNA 表达量也逐渐升高（P＜
0.05）；肝脏和脂肪组织中Toll样受体4 信号通路与胰岛素抵抗指数HOMA-IR 呈显著正相关（P＜0.05），脂肪组织中的相关性显
著高于肝脏组织（P＜0.05）。结论：SGA 生后追赶生长者随年龄增长出现糖脂代谢异常；肝脏和脂肪组织Toll样受体4 信号途径
激活，诱发以TNF-alpha和IL-6 为炎性介质的慢性炎症，促进胰岛素抵抗发生发展；脂肪组织在胰岛素抵抗发生发展中作用更强。 |
| 英文摘要: |
| Objective:To investigate the effect of the toll-like receptor4 (TLR-4) signaling pathway on insulin resistance in small
for gestational age (SGA) rats after birth.Methods:Establishment of the SGA rat model was made by the starvation method in pregnant
rats. Experiment group were divided into three groups: ① Group S1: SGA with catch-up growth; ② Group S2: SGA without catch-up
growth; ③ Group AGA: Appropriate for gestational age (AGA). Blood, liver and omental adipose tissue were collected on the 4th and
12th week after birth. The serum level of tumor necrosis factor-alpha（TNF-alpha）and interleukin-6 (IL-6)were detected by ELISA method. The
concentration of fasting blood-glucose, fasting insulin, free fatty acids, triglyceride, total cholesterol were analyzed. Homeostasis model
assessment for insulin resistance index (HOMA-IR) was calculated. The mRNA expression of TLR-4, myeloid differentiation factor 88
(MyD88), nuclear factor kB (NF-kB), TNF-alpha and IL-6 in liver and omental adipose tissue were evaluated by real-time quantitative
RT-PCR.Results:Along with the growth, the serum level of fasting blood-glucose, fasting insulin, free fatty acids, triglyceride, total
cholesterol, TNF-琢and IL-6in group S1 gradually became higher than that of in group AGA and group S2 (P＜0.05). The mRNA
expression level of TLR-4, MyD88, NF-kB, TNF-alphaand IL-6 in liver and omental adipose tissue in group S1 were remarkably higher than
that of in group AGA and groupS2 (P＜0.05). In groupS1, the TLR-4 signaling pathway was positively related to HOMA-IR. Higher
degree of correlation was observed in omental adipose tissue than in liver (P ＜0.05).Conclusion:Abnormal glucose and lipid
metabolismhappened in SGA rats with catch-up growth with advancing age. Activation of TLR-4 signaling pathway in liver and adipose
tissue induced the releasement of inflammatory cytokines such as TNF-alpha and IL-6, which resulted in chronic inflammatory reaction and
promoted the development of insulin resistance. During the progression of the insulin resistance, the adipose tissue play more important
role. |
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