| 石定 余州 宋雅娟 余震 米萱 苏映军 彭湃.阿魏酸在缺氧条件下促进人脐静脉内皮细胞血管形成的实验研究[J].,2016,16(13):2452-2455 |
| 阿魏酸在缺氧条件下促进人脐静脉内皮细胞血管形成的实验研究 |
| Ferulic Acid Promotes Angiogenesis of HUVECs under Hypoxia |
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| DOI： |
| 中文关键词: 阿魏酸 人脐静脉内皮细胞 缺氧 血管生成 |
| 英文关键词: Ferulic Acid HUVEC Hypoxia Angiogenesis |
| 基金项目:国家自然科学基金面上项目(81272118；30973752) |
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| 中文摘要: |
| 目的：研究阿魏酸（ferulic acid，FA）在缺氧条件下对人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVECs)增
殖、迁移和管腔样结构形成的影响。方法：原代培养人脐静脉内皮细胞，在缺氧实验条件下，细胞被分为7 组，即1 个对照组和6
个实验组。对照组采用1 %酒精处理，实验组用不同浓度（1× 10-8、1× 10-7、1× 10-6、1× 10-5、1× 10-4及1× 10-3mol/L）的阿魏酸处
理。分别采用MTS 法、划痕法、Matrigel 法分析不同浓度阿魏酸处理对人脐静脉内皮细胞的增殖、迁移和管腔样结构形成的影响。
结果：缺氧条件下，浓度为1× 10-6～1× 10-4 mol/L的阿魏酸处理能明显促进HUVECs 的增殖（P<0.05），以1× 10-5mol/L处理的效
果最好（P<0.01）；与对照组相比，1× 10-6mol/L（P<0.05）、1× 10-5 mol/L（P<0.01）及1× 10-4mol/L（P<0.01）阿魏酸处理均能明显促进
HUVECs 横向迁移，以1× 10-5 mol/L 处理迁移的细胞数量最多；1× 10-8～1× 10-4mol/L 阿魏酸处理能不同程度地促进HUVECs
管腔样结构的形成，以1× 10-5mol/L处理形成管腔样结构的数量最多（P<0.01）。结论：阿魏酸在缺氧条件下能促进人脐静脉内皮
细胞的增殖、迁移和管腔样结构形成。 |
| 英文摘要: |
| Objective:To study the effects of ferulic acid (FA) on the proliferation, the migration and the formation of the
tube-like structure of human umbilical vein endothelial cells (HUVECs) under hypoxia.Methods:The primary HUVECs were cultured
under normoxia conditions (21 % O2, 5 %CO2 and 74 %N2), and the experimental conditions of hypoxia (1 % O2, 5 % CO2 and 94 %
N2) were established. Under hypoxia, the cells were divided into seven groups, one control group and six experimental groups. The control
group was treated with 1 % ethanol, and the experimental groups were treated with different concentrations of ferulic acid (1× 10-8,
1× 10-7, 1× 10-6, 1× 10-5, 1× 10-4 and 1× 10-3mol/L). The MTS assay was used to evaluat the effects of the proliferation activity of ferulic
acid on HUVECs after 48 hours; the wound healing assay was used to detect the effects of the lateral migration ability of ferulic acid on
HUVECs after 24 hours; the tube formation assay on Matrige was used to detect the effects of the tube-like structure formation ability of
ferulic acid on HUVECs after 7 hours. SPSS 17.0 was used for statistical analysis. The experimental data of each group were statistically
analyzed by Dunnett-t test, and P<0.05 was statistically different.Results:Under hypoxia, the concentrations of ferulic acid between 1×
10-8 to 1× 10-5 mol/L could promote the proliferationon of HUVECs in a concentration dependent manner, and the effects of 1× 10-5
mol/L were the best. Compared with the control group , the differences were statistically significant (1× 10-6 and 1× 10-4mol/L, P<0.05;
1× 10-5mol/L,p<0.01). Compared with the control group, ferulic acid could promote the migration of HUCECs, and the differences were
statistically significant (1× 10-6mol/L P<0.05; 1× 10-5 mol/L and 1× 10-4mol/L, P<0.01). Ferulic acid had the ability to enhance tube-like
structure formation of HUVECs, and compared with the control group, the differences were statistically significant (1× 10-4mol/L, P<0.
05; 1× 10-5 mol/L, P<0.01).Conclusion:Ferulic acid can promote the proliferation, the migration and the formation of the tube-like
structure of human umbilical vein vascular endothelial cells under hypoxia. |
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