| 黎柏源 王茜云 赵永博 白万胜 胡彪 孙平安 吕书革.VEGF和SDF-1 的协同作用对内皮祖细胞增殖与迁移的影响[J].,2016,16(11):2049-2053 |
| VEGF和SDF-1 的协同作用对内皮祖细胞增殖与迁移的影响 |
| Synergistic Effect of VEGF and SDF-1 on the Proliferation and Migration ofEndothelial Progenitor Cells |
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| DOI： |
| 中文关键词: 血管内皮生长因子 机制衍生因子-1 内皮祖细胞 高血压脑出血 |
| 英文关键词: Vascular endothelial growth factor Stromal derived factor-1 Endothelial progenitor cell Hypertensive intracerebral
hemorrhage |
| 基金项目: |
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| 中文摘要: |
| 目的：研究血管内皮生长因子（VEGF）和基质衍生因子-1（SDF-1）的协同作用对高血压脑出血患者内皮祖细胞（EPCs）增殖
迁移能力的影响。方法：采集急性期高血压脑出血患者与健康对照的外周静脉血，分离培养外周血中的EPCs。用分别含有不同
VEGF 与SDF-1 的培养基处理患者外周血分离的EPCs，Western blot 检测各组细胞以及患者和对照中VEGF和SDF-1 的蛋白表
达。MTT 法检测细胞增值能力，Transwell 法检测细胞迁移能力，分别转染VEGF-siRNA与SDF-1-siRNA 至各组细胞观察抑制
VEGF和SDF-1 对EPCs 增殖迁移能力的影响。结果：VEGF 和SDF-1 在患者中的表达显著高于健康对照组。VEGF和SDF-1 对
EPCs 的增殖和迁移能力有促进作用，且在其协同作用下效果显著。抑制VEGF或SDF-1 显著降低VEGF和SDF-1 对EPCs增殖
迁移能力的促进作用。结论：VEGF和SDF-1的协同作可促进急性期高血压脑出血患者EPCs 的细胞增殖能力和迁移能力，对患
者血管修复提供新的治疗方向。 |
| 英文摘要: |
| Objective:To investigate the synergistic effect of vascular endothelial growth factor (VEGF) and stromal derived factor-
1 (SDF-1) on the proliferation and migration of endothelial progenitor cells (EPCs) in patients with hypertensive intracerebral hemorrhage.Methods:The endothelial progenitor cells were isolated from peripheral venous blood in patients with hypertensive intracerebral
hemorrhage and healthy controls. EPCs isolated from peripheral venous blood in patients were treated with medium contain VEGF and
SDF-1 respectively. The protein expression levels of VEGF and SDF-1 were detected by Western blot method analysis. Cell proliferation
was measured by MTT assay. Cell migration was determined by Transwell method. The effect of VEGF or SDF-1 inhibition on cell proliferation
and migration was achieved by VEGF-siRNA or SDF-1-siRNA transfection.Results:The expression level of VEGF and SDF-1
was significantly increased in EPCs from patients as compared with that from healthy controls. After treatment with VEGF or SDF-1, the
cell proliferation and cell migration ability of EPCs were promoted significantly. In addition, the promoting effects were more significant
with synergistic action of VEGF and SDF-1. Moreover, inhibition of VEGF or SDF-1 significantly decreased the promoting effects of
VEGF and SDF-1 on cell proliferation and migration.Conclusion:VEGF and SDF-1 exerted significant synergistic effects on the cell
proliferation and migration ability of EPCs, which may provide a new therapeutic direction for the vascular repair in hypertensive intracerebral
hemorrhage. |
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