| 梁小燕 李洪霞 王玉 左震华 蔡少华.辛伐他汀对急性肺损伤及囊性纤维化跨膜传导调节体表达的影响[J].,2016,16(11):2037-2040 |
| 辛伐他汀对急性肺损伤及囊性纤维化跨膜传导调节体表达的影响 |
| Role of Simvastatin in Rats with Acute Lung Injury and Expression of CysticFibrosis Membrane Conductance Regulator |
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| DOI： |
| 中文关键词: 辛伐他汀 急性肺损伤 囊性纤维化跨膜传导调节体 |
| 英文关键词: Simvastatin Acute lung injury Cystic fibrosis transmembrance conductance regulator |
| 基金项目:国家自然科学基金项目（81200059） |
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| 中文摘要: |
| 目的：探讨辛伐他汀对急性肺损伤大鼠囊性纤维化跨膜传导调节体（CFTR氯离子通道）的影响及其对减轻急性肺损伤的作
用。方法：40只雄性SD大鼠随机分为空白组、模型组、辛伐他汀低剂量组（20 mg/kg）、辛伐他汀中剂量组（40 mg/kg）、辛伐他汀高
剂量组（80 mg/kg）；气道内滴注脂多糖（10 mg/kg）制备急性肺损伤模型。进行肺湿/干重比、肺泡灌洗液蛋白检测，HE 染色观察肺
组织的病理变化；实时荧光定量PCR 检测肺组织匀浆CFTR mRNA 表达。结果：结果显示，模型组的肺湿干重比，肺泡灌洗液蛋白
较空白组高（P<0.05），病理示肺泡膈增厚，大量炎性细胞浸润，肺泡腔内可见红细胞及血肿，提示模型复制成功。辛伐他汀低剂量
组的肺湿/干重比、肺泡灌洗液蛋白与模型组相比无明显差异，病理可见肺损伤较重，与模型组相比无改善；CFTR mRNA表达与
模型组相比稍高但无明显差异（P<0.05）。辛伐他汀中高剂量组中肺湿/ 干重比、肺泡灌洗液蛋白与模型组相比有所降低，肺组织
CFTRmRNA表达较模型组明显增加（P<0.05），但中高剂量组之间无明显差异（P>0.05）；病理可见肺泡膈增厚，极少见炎性细胞浸
润及透明膜，肺泡腔内未见明显出血和水肿，肺损伤程度较模型组减轻。结论：中高剂量的辛伐他汀（>40 mg/kg）对急性肺损伤有
一定保护作用，并上调CFTR 的表达。 |
| 英文摘要: |
| Objective:To investigate the effect of simvastatin on the expression of cystic fibrosis transmembrane conduction regulating
body (CFTR chloride ion channels) in rats with acute lung injury, and its role in alleviating the acute lung injury.Methods:40 male
SD rats were randomly divided into blank group, ALI model group, low dose simvastatin group (20 mg/kg), middle dose simvastatin
group (40 mg/kg) and high dose simvastatin group (80 mg/kg); We established a rat model of acute lung injury by intratracheal instillation
of lipopolysaccharide (LPS) with a dose of 10 mg/kg. The wet-dry (W/D) ratio of the lung, total protien in the bronchoalveolar lavage
fluid (BALF) were examined, HE statining were used to observe the pathological changes in lung tissue of rats; and the expression of
CFTR mRNA in lung tissues was determined with quantitative RT-PCR (Q-PCR).Results:The results showed that the W/D of lung and
total protein of BALF in ALI model group were higher than those in blank group (P<0.05); In pathological examination of lung tissue,
ALI model group was characterized by a wider alveolar septum, a large number of inflammatory cell infiltration, alveolar wall swelling
and destruction, a large number of edema and hemorrhage in lung parenchyma. Those results showed that the model of acute lung injury
was established. Compared with ALI model group, the W/D of lung, total protein of BALF had no significantly difference in low dose
simvastatin group (P>0.05); pathological examination of lung tissue injury did not show significant difference. Moreover, CFTR mRNA
expression in low dose simvastatin group was slightly higher than ALI model group (P>0.05). In the middle and high dose simvastatin
groups, the W/D of lung and total protein of BALF were lower than those in ALI model group (P<0.05), while CFTR mRNA expression
was remarkably higher than that in ALI model group (P<0.05); pathological examination revealed that the lung tissue injury in middle
and high dose simvastatin groups were milder than that in ALI model group. In pathological examination, The middle and high dose simvastatin groups showed alveolar septum thickening. However, It is hardly to see inflammatory cell infiltration and hyaline membranes in
lung parenchyma, alveolar edema and hemorrhage can not be seen in those groups as well. Furthermore, middle dose simvastatin group
has no difference fromhigh dose simvastatin group in CFTR mRNAexpression (P>0.05).Conclusion:Middle and high dose (>40 mg/kg)
simvastatin can ameliorate acute lung injury by lipopolysaccharide (LPS), this may relate with up-regulating CFTRmRNA expression. |
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