| 刘道洲 刘苗 蔡容巧 成颖 张邦乐 周四元△.转铁蛋白修饰磁性纳米粒的制备及其体外抗肿瘤活性研究[J].,2016,16(9):1631-1635 |
| 转铁蛋白修饰磁性纳米粒的制备及其体外抗肿瘤活性研究 |
| Preparation and in Vitro Anti-tumor Activity of Transferrin ModifiedMagnetic Nanoparticle |
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| DOI： |
| 中文关键词: 转铁蛋白 磁纳米粒 阿霉素 |
| 英文关键词: Transferrin Magnetic nanoparticle Doxorubicin |
| 基金项目:陕西省科技统筹创新工程计划项目（2012KTCL03-12） |
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| 中文摘要: |
| 目的：本研究旨在构建一种转铁蛋白修饰负载阿霉素（DOX）的磁纳米粒靶向递药系统，以提高阿霉素作用的靶向性。方法：
采用化学共沉淀法制备转铁蛋白修饰负载阿霉素的磁性纳米粒（DOX@MNP），采用zeta 电位及纳米粒度分析仪测定
DOX@MNP 的粒径及其zeta 电位，透析法评价DOX@MNP的体外释药特征。通过MTT 实验，研究DOX@MNP 与游离DOX对
A549细胞的细胞毒性，通过激光共聚焦显微镜和流式细胞仪观察A549 细胞对DOX@MNP 与游离DOX 的摄取情况。结果：
DOX@MNP 的释药具有pH 依赖性。MTT实验结果显示，DOX@MNP 与游离DOX 具有相当的细胞毒性；激光共聚焦显微镜和
流式细胞仪检测结果显示A549 细胞对DOX和DOX@MNP 的摄取没有明显差异。结论：本文构建了一种转铁蛋白修饰包载阿
霉素的磁纳米粒，体外结果显示其具有与游离DOX相当的细胞毒性，为进一步进行体内实验奠定了基础。 |
| 英文摘要: |
| Objective:This study aimed to construct a transferrin modified magnetic nanoparticle, which can targeted deliver doxorubicin(
DOX) to tumor cells.Methods:The magnetic nanoparticle(DOX@MNP) was prepared by the chemical co-precipitation. The zeta
potential and particle size were detected by Laser Particle Size Analyzer. The dialysis method was used to evaluate the characteristic of
drug release of DOX lipid hybrid nanoparticle. The anti-tumor activity of DOX@MNP was studied by MTT method. The cell uptake of
DOX@MNP in A549 cells was observed with flow cytometry and confocal laser scanning microscope.Results:The drug release of
DOX@MNP exhibited an acidic sensitivity property. DOX@MNPshowed the same cytotoxicity as DOX didon A549 cells. The flow cytometry
and confocal laser scanning microscope results showed that there was no significant difference in cellular uptake between DOX
and DOX@MNP in A549 cells.Conclusion:This study constructed a magnetic nanoparticle modified with transferrin to deliver DOX to
tumor cells. DOX@MNP had the same anti-tumor activity as free DOX in vitro. DOX@MNP is worthy of investigation in vivo. |
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