杨丽媛 孙姝 逄明杰 郭菲菲 孙向荣 公衍玲 徐珞.大鼠室旁核注射orexin-A对体重的影响[J].,2016,16(8):1450-1453 |
大鼠室旁核注射orexin-A对体重的影响 |
Effect of Orexin-A Microinjected into the Paraventricular Nucleus ofHypothalamus on BodyWeight of Rats |
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DOI: |
中文关键词: Orexin-A PVN 非运动性活动产热 负能量平衡 体重 |
英文关键词: Orexin-A PVN Non-exercise activity thermogenesis Negative energy balance Body weight |
基金项目:国家自然科学基金项目(81470815,31071014,81100260,81270460,81300281,81500414);
山东省优秀中青年科学家科研奖励基金项目(BS2014YY009);青岛市科技局项目(13-1-4-170-jch,14-2-3-3-nsh) |
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中文摘要: |
目的:探讨大鼠室旁核(PVN)注射orexin-A对体重的影响。方法:大鼠室旁核(PVN)微量注射orexin-A,用大脑置管埋管、组
织化学染色等方法探讨PVN 注射orexin-A 对其体重的影响。结果:与安慰剂组大鼠相比,PVN 注射orexin-A 组大鼠体重明显减
轻(P<0.05),而orexin-A 组和安慰剂组摄食量无明显差异(P>0.05)。注射结束后6 天,orexin-A 处理大鼠的体重仍显著低于注射
前(P<0.05),而安慰剂组大鼠则比注射前显著增重(P<0.05)。药物注射可显著降低机体脂肪,但并不特异存在于注射orexin-A 或
安慰剂的大鼠身上。Orexin-A 组和安慰剂组大鼠的肌肉量和脂肪量均显著降低(P<0.05),但注射orexin-A 的大鼠降低更明显。与
安慰剂组相比,orexin-A 处理后的摄食转化率显著降低(P<0.05)。结论:大鼠室旁核(PVN)注射orexin-A 可通过增加活动量产生
负能量平衡,引起体重减轻。 |
英文摘要: |
Objective:To investigate the effect of orexin-A microinjected into the paraventricular nucleus of the hypothalamus
(PVN) on body weight in rats.Methods:Chronic guide cannulae were implanted into PVN of rats. Orexin-A was microinjected into the
brain site. The effect of orexin-A on body weight in rats was detected.Results:The loss of body weight in orexin-A-treated rats was more
significantly than their vehicle-injected counterparts(P<0.05), but they had no difference in food intake (P>0.05). The rats were more
significantly active after intra-PVN orexin microinjection compared to vehicle(P<0.05)Conclusion:Orexin-A microinjected into the
paraventricular nucleus of the hypothalamus (PVN) can induce weight loss in rats through producing negative energy balance via the
increase of physical activity. |
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