| 仇生龙 蔡冬兰 陈桂明 王敏 严东旺.MT1F mRNA在结肠癌组织中的表达及其临床病理意义[J].,2016,16(7):1257-1261 |
| MT1F mRNA在结肠癌组织中的表达及其临床病理意义 |
| Expression of MT1F mRNA in Colon Cancer and Its ClinicopathologicalSignificance |
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| DOI： |
| 中文关键词: MT1F 结肠癌 临床病理意义 |
| 英文关键词: MT1F Colon cancer Clinicopathological Significance |
| 基金项目:国家自然科学基金项目(81172328)；上海市科委医学引导项目(124119a1700) |
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| 中文摘要: |
| 目的：探讨MT1F mRNA 在结肠癌组织中的表达及其临床病理意义。方法：采用TaqMan 探针实时荧光定量Real-time PCR
检测40 例结肠癌及对应正常粘膜组织中MT1F mRNA的表达，并通过免疫组化检测Metallothionein(MT)的蛋白表达。结果：82.5
%(33/40 例)的结肠癌组织MT1F mRNA表达较对应正常组织明显下调，降低2.4 倍至113 倍不等。MT1F mRNA水平与结肠癌
患者的年龄、性别、肿瘤部位、肉眼形态、直径、分化及Dukes分期无关。在MT1F mRNA 低表达的病例中，癌组织MT 蛋白表达低
于对应正常组织(P<0.05)。结论：结肠癌组织MT1F mRNA水平显著下调，但与结肠癌的临床病理特征无关。 |
| 英文摘要: |
| Objective:To explore the expression of MT1F mRNA in colon cancerous tissues, and analyze its clinicopathological
significance.Methods:TaqMan quantitive Real-time PCR was performed to detect the expression of MT1F mRNA in 40 paired samples
with primary tumor and adjacent normal mucosa. Metallothionein (MT) protein was measured by immunohistochemistry.Results:MT1F
mRNA was significantly downregulated in 82.5% (33/40) tumorous tissues compared with normal colonic mucosa, and the expression
ratio ranged from2.4 to 113. The expression levels of MT1F mRNA did not correlate to age, gender, tumor location, morphous, diameter,
differentiation and Dukes stages of colon cancer patients. Immunohistochemistry showed that low levels of MT protein were also existed
in patients with MT1F mRNA downregulation.Conclusion:MT1F mRNA was obviously downregulated in colon cancers, but was not
associated with clinic pathological characters of colon cancer. |
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