| 李晓丽 孔宪明△ 徐凌云 薛海燕 曹兰芳.可溶性PD-1 和调节性T细胞在肺炎支原体感染和哮喘的实验研究[J].,2016,16(7):1205-1208 |
| 可溶性PD-1 和调节性T细胞在肺炎支原体感染和哮喘的实验研究 |
| sPD-1 and Regulatory T Cells in Asthma and Mycoplasma PneumoniaeInfection |
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| DOI： |
| 中文关键词: 哮喘 肺炎支原体 sPD-1 调节性T细胞 |
| 英文关键词: Asthma Mycoplasma Pneumoniae sPD-1 Regulatory T cells |
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| 中文摘要: |
| 目的：探讨可溶性PD-1（soluble PD-1，sPD-1）和调节性T 细胞（Regulatory T lymphocyte，Treg）在肺炎支原体（mycoplasma
pneumoniae，MP）感染与哮喘模型小鼠关系中的作用。方法：20 只BALB/c 小鼠随机分为4 组（n=5），其中2 组建立鸡卵白蛋白
（ovalbumin，OVA）致敏哮喘模型，将MP菌液滴入其中1 组OVA 小鼠和1 组对照小鼠，形成OVA 致敏并MP 感染组，OVA 致
敏组，MP 感染组及正常对照组。采用酶联免疫吸附试验（enzyme linked immunosorbent assay,ELISA)方法分别检测各组小鼠血清、
肺泡灌洗液(Bronchoalveolar Lavage Fluid，BALF)中sPD-1 的浓度；采用流式细胞术检测脾脏组织中Treg细胞的比例。结果：①与
正常对照组相比，OVA致敏并感染MP 组、单纯OVA致敏组和单纯MP 感染组小鼠血清sPD-1 的浓度均升高，差异有统计学意
义（P<0.05），BALF中的sPD-1 的浓度亦增高，但无统计学差异；②与正常对照组相比，OVA 致敏并感染MP 组、单纯OVA 致敏
组小鼠的脾脏Treg细胞占淋巴细胞的比例下降，而单纯MP 感染组Treg细胞的比例升高，差异具有统计学意义（P<0.05）。结论：
sPD-1和Treg 细胞可能参与了哮喘小鼠感染MP的发病。 |
| 英文摘要: |
| Objective:Discussion whether sPD-1（soluble PD-1）and Treg cells(regulatory T cells) be involved allergic model and
Mycoplasma pneumoniae infection.Methods:20 BALB/c mice were randomly divided into 4 groups, two groups of which were sensitized
and challenged with OVA. One of OVA mice groups and one of control groups were inoculated intranasally with MP. We detected
the concentration of sPD-1 in serum and bronchoalveolar lavage with Enzyme-linked immunosorbent assay. The proportion of regulatory
T cells in the spleen was detected by flow cytometry.Results:① Compared with the control group,the concentration of sPD-1 in serum
and bronchoalveolar lavage in other goups were increased, and the concentration of sPD-1 in serum was statistically significant(P<0.05).
②Compared with normal mice, the proportion of regulatory T cells in the spleen of groups which estabilished allergic airway and estabilished
allergic airway with MP infectin were decreased, the difference between groups was statistically significant (P <0.05 ).Conclusion:Elevated sPD-1 and reduction of Treg may be involved in the relation between asthma and Mycoplasma pneumoniae infection. |
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