林琳 李阳 刘云奇 张文波 殷胜利.CTSB 对人脐静脉血管内皮细胞系增殖及凋亡的影响[J].,2016,16(4):611-615 |
CTSB 对人脐静脉血管内皮细胞系增殖及凋亡的影响 |
Influence of CTSB on Proliferation and Apoptosis of Human Umbilical Vein Endothelial Cell |
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DOI: |
中文关键词: 组织蛋白酶B 人脐静脉血管内皮细胞系 增殖 凋亡 |
英文关键词: Cathepsin B Human umbilical vein endothelial cell Proliferation Apoptosis |
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中文摘要: |
目的:探索组织蛋白酶B(CTSB)对人脐静脉血管内皮细胞系(HUVEC)增殖及凋亡的影响。方法:采用细胞转染法得到慢病
毒空载体转染HU-MOCK 细胞及CTSB 过表达的HU-CTSB 细胞,与正常的HUVEC 细胞进行比较。采用MTT法检测HUVEC
增殖情况;流式细胞仪检测各组细胞凋亡情况;采用蛋白免疫印迹法检测Bal-2/ Bax 及Caspase 家族(Caspase-3/9)的表达情况。结
果:HU-CTSB组各时间点HUVEC 的增殖率明显低于其他两组,且差异具有统计学意义(P<0.05);HU-CTSB组的HUVEC 早期
凋亡率(右下象限)和晚期凋亡率(右上象限)显著高于HUVEC组和HU-MOCK 组,且组间差异具有统计学意义(P<0.05);
HU-CTSB组的抑制凋亡蛋白Bcl-2表达量明显低于其他两组,且促凋亡蛋白Bax表达量显著高于其他两组,差异均具有统计学
意义(P<0.05);HU-CTSB 组caspases-3 和caspases-9 的活化片段显著高于其他两组,差异均具有统计学意义(P<0.05)。结论:
CSTB 通过激活Caspase-3/9 信号通路来上调Bax,并下调Bal-2,显著抑制HUVEC的增殖,并促进其凋亡,进而影响心血管疾病
的发展。 |
英文摘要: |
Objective:To explore the influence of cathepsin B (CTSB) on proliferation and apoptosis of human umbilical vein
endothelial cell (HUVEC).Methods:Empty lentivirus vector transfection HU-MOCK cells and HU-CTSB cells from CTSB over
expression cells were obtained by cell transfection method, which were compared with normal HUVEC cells. The HUVEC proliferation
status was detected by MTT method; the cell apoptosis status was detected by flow cytometry instrument; and the expressions of
Bal-2/Bax and Caspase-3/9 were detected by protein immunoblot method.Results:The HUVEC proliferation rate of HU-CTSB group
was significantly lower than that of the other two groups at each time point, the differences were statistically significant (P<0.05). The
HUVEC early apoptosis rate (lower right quadrant) and late apoptosis rate (upper right quadrant) of HU-CTSB group were significantly
higher than those of HUVEC and HU-MOCK groups, the differences were statistically significant (P<0.05). The expression of inhibiting
apoptosis protein Bcl-2 in HU-CTSB group was significantly lower than that of the other two groups, while the expression of promoting
apoptosis proteins Bax in HU-CTSB group was significantly higher than that of the other two groups, the differences were statistically
significant (P<0.05). The activation pieces of caspases-3 and caspases-9 of HU-CTSB group were significantly higher than the other two
groups, the differences were statistically significant (P<0.05).Conclusion:CTSB can raise Bax and cut down Bal-2 by activating the
signaling pathways of Caspase-3/9, which will remarkably inhibit the proliferation, promote the apoptosis of HUVEC, and influence the
development of cardiovascular disease. |
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