| 贾耿 夏锡伟 支枫 彭亚 邵耐远 王强 王穗暖 杨伊林.土贝母苷甲经miR-21/ PDCD4 途径诱导胶质瘤U251细胞凋亡[J].,2016,16(3):419-422 |
| 土贝母苷甲经miR-21/ PDCD4 途径诱导胶质瘤U251细胞凋亡 |
| Tubeimoside-1 Induces Apoptosis in Human Glioma Cell U251 ThroughmiR-21/PDCD4 Pathway |
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| DOI： |
| 中文关键词: 土贝母苷甲 胶质瘤 凋亡 miR-21 PDCD4 |
| 英文关键词: Tubeimoside-1 Glioma Apoptosis miR-21 PDCD4 |
| 基金项目:国家自然科学基金项目（31071046; 81302197）；常州市社会发展项目（CS20092015; CS20102010）；
常州市卫生局重大课题（ZD200903; ZD201007）；常州市科技局指导项目（CY20119004） |
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| 中文摘要: |
| 目的：研究土贝母苷甲（TBMS I）对胶质瘤U251细胞的抗肿瘤作用以及探究土贝母苷甲对miR-21 及其靶基因PDCD4 基
因表达的影响。方法：U251 细胞在体外进行培养，四甲基偶氮唑盐（MTT）法检测不同浓度的土贝母苷甲对细胞增殖的影响；
Hoechst33258 染色观察细胞核形态的变化；实时荧光定量PCR 检测miR-21 和PDCD4基因的表达情况；Western blot 检测PDCD4
蛋白的表达情况。结果：土贝母苷甲能够显著抑制U251 细胞的增殖，其抑制作用呈剂量和时间依赖性。Hoechst33258 染色观察到
土贝母苷甲处理组细胞的细胞核形态表现出典型的凋亡特征。PCR结果显示：随着土贝母苷甲浓度增加，miR-21 的表达逐渐降低
（P<0.05），PDCD4 基因表达显著增加（P<0.05）。Western blot 结果提示：与阴性对照组相比，15、30 滋g/mL 土贝母苷甲显著上调了
PDCD4 蛋白的表达（P<0.05）。结论：土贝母苷甲能够显著抑制人胶质瘤U251细胞的增殖并诱导细胞发生凋亡，其机制可能与下
调miR-21 的表达和上调PDCD4 的表达有关。 |
| 英文摘要: |
| Objective:To study the antitumor effect of tubeimoside-1 on human glioma U251 cell line and explore the influence of
tubeimoside-1 on the expression levels of miR-21 and programmed cell death4 (PDCD4).Methods:U251 cells were cultured in vitro
and treated with different concentrations of tubeimoside-1, cell viability was evaluated using MTT assay. Cell apoptosis was observed by
Hoechst 33258 staining with a fluorescence microscope. The expression levels of miR-21 and PDCD4 mRNA were detected by real-time
fluorescent quantitative PCR. Western blot analysis was used to detect the expression of PDCD4.Results:Tubeimoside-1 inhibited the
proliferation of the U251 cells in a dose- and time-dependent manner. Cells exposed to tubeimoside-1 showed obviously changes of cellular
ckaryomorphism which indicated apoptosis. Moreover, PCR analysis showed that after tubeimoside-1treatment, the expression of
miR-21 tended to decrease gradually (P<0.05）, down-regulation of miR-21 in U251 cell line resulted in increased expression of PDCD4
（P<0.05）. The expression of PDCD4 has also been enhanced by tubeimoside-1 (15 and30 ug/mL) using Western blot analysis（P<0.05）Conclusion:Tubeimoside-1 significantly suppressed proliferation and induced apoptosis of U251 cells, the mechanismmay be related to
the down-regulation of miR-21 and up-regulation of PDCD4. |
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