| 赵海军 王双双 张瑶 张彤帅 王丹丹 徐悦 马一鸣 王广友.高盐环境对小鼠脑缺血后干扰素诱导蛋白10 表达的影响[J].,2016,16(1):21-24 |
| 高盐环境对小鼠脑缺血后干扰素诱导蛋白10 表达的影响 |
| Effects of Excess Salt Intake on the Expression of IP-10 after CerebralIschemia |
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| DOI： |
| 中文关键词: 脑缺血 高盐 胶质细胞 干扰素诱导蛋白10（IP-10） |
| 英文关键词: Cerebral ischemia High salt Glial cells Interferon induced protein 10 (IP-10) |
| 基金项目:国家自然科学基金项目（81070943，31371079) |
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| 中文摘要: |
| 目的：探讨高盐环境对小鼠脑缺血后IP-10（Interferon induced protein 10，IP-10）表达的影响。方法：将C57BL/6 雄性小鼠分
为高盐饮食（4%NaCl 鼠粮和1%NaCl 水）组和正常饮食组，建立永久性大脑中动脉结扎（permanent middle cerebral artery ligation，
pMCAL）小鼠模型；激光共聚焦显微镜检测脑缺血组织IP-10 表达情况；RT-PCR（Reverse transcription PCR）检测脑缺血组织中
IP-10 表达情况；ELISA 方法检测内皮细胞和神经胶质细胞氧糖剥夺（oxygen-glucose deprivation，OGD）6h 后上清中IP-10 表达。
结果：免疫荧光观察发现高盐饮食组小鼠脑缺血组织中IP-10 阳性细胞表达增加；与正常饮食组相比，高盐饮食组缺血侧脑组织
中IP-10 表达显著增加，有统计学意义（P1d<0.05，P2d<0.001）；与内皮细胞相比，神经胶质细胞OGD后，上清中IP-10 表达显著增
多（P<0.001）。结论：高盐环境促进脑缺血后IP-10 表达；脑缺血损伤过程中，脑内的IP-10 主要是由胶质细胞释放的。 |
| 英文摘要: |
| Objective:To study the effect of excess salt intake on the expression of IP-10 after cerebral ischemia.Methods:The
experimental animal were divided into high salt diet group（4%NaCl rat food and 1%NaCl water）and normal diet group, then established
the permanent middle cerebral artery ligation (pMCAL) model in mice; The expression of IP-10 in ischemic brain tissue was detected
by laser scanning confocal microscope; The expression of IP-10 in cerebral ischemic tissues were detected by reverse transcription
PCR; The expression of endothelial cells and glial cells in the supernatant of oxygen-glucose deprivation (OGD) 6 h were detected by
ELISA.Results:The high salt group has more IP-10 positive cells vie immunofluorescence staining; Compared with the normal diet
group, and the IP-10 expression level of high salt diet group mice was significantly increased by RT-PCR (P1d<0.05, P2d<0.001); Compared
with the endothelial cells group, the IP-10 expression of glial cells was significantly increased in the supernatant after OGD
(P<0.001).Conclusion:Excess salt intake promotes the expression of IP-10 after cerebral ischemia; endothelial cells and glial cells both
express IP-10, and the expression of IP-10 of glial cells was higher than that of endothelial cells. |
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