| 凌琳 顾少华 承燕 丁留成.成体心脏干细胞经静脉移植后在心梗小鼠体内的分布研究[J].,2015,15(36):7024-7027 |
| 成体心脏干细胞经静脉移植后在心梗小鼠体内的分布研究 |
| Distribution of Adult Cardiac StemCells via Intravenous Cell Transplantationin Myocardial Infarction Mouse Model |
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| DOI： |
| 中文关键词: 心脏成体干细胞 细胞移植 心肌梗死 |
| 英文关键词: Adult Cardiac StemCell Cell Transplantation Myocardial Infarction |
| 基金项目:江苏省自然科学青年基金项目（BK20140296）；苏州市科教兴卫项目（KJXW2013004）；
苏州大学校级青年教师自然科学基金项目（SDY2013A29） |
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| 中文摘要: |
| 目的：观察心肌梗死小鼠静脉移植成体心脏干细胞后，细胞在小鼠体内各器官的分布情况。明确心梗后静脉移植成体心脏干
细胞在小鼠体内的分布和归巢情况。方法：分离培养小鼠心脏成体干细胞，采用流式细胞仪鉴定细胞，通过亲脂性染料CM-DiI标
记细胞后行小鼠急性心肌梗死模型建立和细胞移植，分别在细胞移植后7、14、8 天取小鼠心脏、肝脏、脑、脊髓、肺脏，行冰冻切
片，在荧光显微镜下观察移植细胞在各组织器官存活和分布情况。结果：成体心脏干细胞分离培养后呈贴壁生长，流式细胞仪检
测显示细胞纯度>80%。CM-DiI标记后荧光显微镜下观察可见标记的细胞胞浆胞核均被染成呈明亮的红色。心肌梗死后经静脉
移植成体心脏干细胞，细胞在各组织中分布呈变化过程，7 天时，在肺脏和肝脏分布较多，至14 天和28 天时，肺脏和肝脏分布减
少，心脏分布逐渐增多，表现出向心脏的" 归巢" 现象，而脑和脊髓在28 天的观察时间内分布较少。结论：采用CM-DiI标记心
脏成体干细胞，操作简单，标记效果好，可用于短期的细胞体内追踪。小鼠心肌梗死后行经静脉成体心脏干细胞移植，28 天后细胞
在心脏的分布逐渐增多，表现出向心脏的" 归巢" 现象。 |
| 英文摘要: |
| Objective:This study was to investigate the distribution of adult cardiac stem cell after intravenous cell transplantation
in a mouse myocardial infarction model.Methods:Adult cardiac stem cells were isolated and cell purity was assessed. After labeled by
CM-DiI, cells were transplanted via venous to myocardial infarction mouse model. Tissues including heart, liver, brain, spine and lung
were isolated at different time point after cell transplantation and CM-DiI positive cells were assessed.Results:Adult cardiac stem cells
were isolated successfully with a cell purity of more than 80%. CM-DiI labeled adult cardiac stem cell showed bright red under
fluorescent microscopy. 7 days after cell transplantation, most cells were found in liver and lung. However, 14 days and 28 days
observation found more transplanted cells in heart while cells in liver and lung decreased. There were few cells in brain and spine.Conclusion:CM-DiI can be used to label adult cardiac stem cell and cell tracking in vivo. Intravenous cell transplantation in myocardial
infarction mouse model showed cell homing to the heart 28 days after transplantation. |
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