| 沙鑫 李锋 吴太林 马文瑞 李寰 陶惠人.丹参素抑制RANKL诱导的破骨细胞分化[J].,2015,15(36):7017-7020 |
| 丹参素抑制RANKL诱导的破骨细胞分化 |
| Danshensu Suppresses RANKL-induced Osteoclastogenesis in Vitro |
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| DOI： |
| 中文关键词: 丹参素 核激活因子kB 受体 破骨细胞 细胞培养 |
| 英文关键词: Danshensu RANKL Osteoclast Cell culture |
| 基金项目:国家自然科学基金项目（81170256；81070698） |
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| 中文摘要: |
| 目的：研究丹参素对RANKL诱导的破骨细胞分化的影响。方法：运用冲洗法从股骨、胫骨中获得小鼠骨髓源性单核巨噬细
胞用于体外RANKL 诱导的破骨细胞分化，同时，施加不同剂量的丹参素干预，经TRAP染色法在形态学上观察观，蛋白印迹法检
测蛋白水平的变化，实时定量PCR 检测mRNA 水平变化来研究丹参素对RANKL诱导的骨髓源单核巨噬细胞破骨分化的影响。
结果：①不同剂量丹参素干预组与对照组相比，TRAP 阳性破骨细胞数量得到了明显抑制（P<0.05）。②不同剂量丹参素干预组与
对照组相比，磷酸化Akt的上调量被明显的降低。磷酸化p38 MAPK，JNK和ERK 的变化则不明显。③不同剂量丹参素干预组与
对照组相比，c-fos，TRAP，CTSK 等参与破骨细胞分化的重要基因表达减少，NFATc1 变化不明显。结论：丹参素通过下调磷酸化
Akt水平的途径抑制了RANKL诱导的破骨细胞分化。 |
| 英文摘要: |
| Objective:To evaluate the effects of Danshensu (DSS) on the RANKL-induced osteoclastogenesis and the mechanism
in vitro.Methods:Bone marrow cells were obtained by flushing the femurs and tibiae of mice, the non-adherent cells were collected to
differentiate in the presence or absence of Danshensu and RANKL. Count the number of the TRAP+ multinucleated osteoclasts, western
bolt analysis and quantitative real-time PCR analysis.Results:①The number of osteoclast decreased comparing differernt dose danshensu
group with control group. ② The phosphorylation of Akt upregulated by RANKL-induced degraded by danshensu with different dose
comparing to control group, while no significant difference were found in phosphorylation of ERK, p38 MAPK and JNK.③We found the
expression of mRNA of the osteoclastic marker genes (TRAP, CTSK) were attenuated by addition of danshensu comparing to control
group, as well as c-fos, but not seen in NFATc1.Conclusion:Danshensu suppress RANKL-induced osteoclastogenesis by downgrading
phosphorylation of Akt. |
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