| 王桂明 杨婵 鲁阳芳 谷雪 王彦 闫超.去氢木香内酯干预人乳腺癌MCF-7细胞机制的探究[J].,2015,15(34):6601-6605 |
| 去氢木香内酯干预人乳腺癌MCF-7细胞机制的探究 |
| Study on Mechanismof Dehydrocostus Lactone Treatment on Breast CancerMCF-7 Cells |
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| DOI： |
| 中文关键词: MCF-7细胞 去氢木香内酯 细胞凋亡 细胞代谢组学 线粒体跨膜电位 |
| 英文关键词: MCF-7 cells Dehydrocostus lactone Apoptosis Cell metabonomics MMP |
| 基金项目:国家自然科学基金项目（21175092, 21105064）；国家重大科学仪器设备开发专项（2011YQ150072, 2011YQ15007204,
2011YQ15007207, 211YQ15007210）；上海市自然科学基金项目（12ZR1413600） |
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| 中文摘要: |
| 目的：探讨去氢木香内酯对乳腺癌MCF-7 细胞凋亡、线粒体跨膜电位及代谢物的影响，为研究去氢木香内酯诱导MCF-7 细
胞凋亡的作用机制提供新的视角。方法：采用流式细胞仪测定不同浓度去氢木香内酯(0、2、4、8 滋g/mL）对MCF-7细胞凋亡及线粒
体跨膜电位的影响；GC-TOFMS测定去氢木香内酯作用前后，MCF-7细胞内具有显著性变化的代谢差异物。结果：研究结果表明，
去氢木香内酯能诱导MCF-7 细胞的凋亡、促进线粒体跨膜电位的降低；正交偏最小二乘法判别分析（OPLS-DA）多维统计方法对
代谢组学数据分析得到柠檬酸、D-核糖、脯氨酸、苯丙氨酸、赖氨酸等16 种代谢差异物。结论：推测去氢木香内酯通过引起线粒体
跨膜电位降低而破坏了线粒体的结构，进一步阻碍了线粒体的功能，导致了细胞内代谢物的紊乱，最终诱导了细胞的凋亡。 |
| 英文摘要: |
| Objective:This study aims to investigate the effects of dehydrocostus lactone on apoptosis, mitochondrial membrane
potential (MMP) and intracellular metabolites of MCF-7 cells and to explore the mechanism of apoptosis promoted by Dehydrocostus
lactone.Methods:In vitro experiments, the effects of dehydrocostus lactone with different concentrations (i.e. 0, 2, 4, 8 ug/mL) on the
apoptosis and MMP of MCF-7 cells were investigated by flow cytometry. Furthermore, we examined the differences in metabolic profiles
between control and drug-treated with GC-TOFMS.Results:Significant induction of apoptosis was detected in MCF-7 cells. Moreover,
dehydrocostus lactone could also promote the decline of MMP. Orthogonal partial least squares discriminant analysis (OPLS-DA) was
used to process the metabolic data. A total of sixteen metabolites such as citric acid, D-ribose, L-proline, phenylalanine, L-lysine were
preliminarily identified as differential metabolites.Conclusion:These results indicated that dehydrocostus lactone may destroy the
mitochondria structure by disrupting MMP. The destroyed mitochondrial disturbed the balance of metabolites in MCF-7 cells and thus
induced the apoptosis of MCF-7 cells. |
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