| 何珊珊 李锐 吴迪 王中恒 彭正午 王化宁 谭庆荣1.天麻素对脑缺血再灌注小鼠海马新生神经元的保护作用[J].,2015,15(32):6241-6244 |
| 天麻素对脑缺血再灌注小鼠海马新生神经元的保护作用 |
| Protective Effects of Gastrodin on the Hippocampal Newborn Neurons afterCerebral Ischemia-Reperfusion |
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| DOI： |
| 中文关键词: 天麻素 大脑中动脉闭塞 DCX |
| 英文关键词: Gastrodin MCAO DCX |
| 基金项目:国家自然科学基金项目（81371478，81201041，81401109） |
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| 中文摘要: |
| 目的：探讨不同剂量天麻素（Gastrodin，GAS）对缺血再灌注模型小鼠海马新生神经元的保护作用及其可能机制。方法：将50
只C57BL/6小鼠随机分为假手术组（Sham）、模型组（MCAO+Vehicle）、模型+天麻素低剂量(10 mg/kg）组（MCAO +GAS（L）），模
型+ 天麻素中剂量（50 mg/kg）组（MCAO + GAS（M）），模型+ 天麻素高剂量（100 mg/kg）组（MCAO +GAS（H））。除Sham 组接受
假手术外（皮肤切开，分离颈动脉），分别对MCAO组及MCAO +GAS各组行右侧颈内动脉栓线术，造成并保持大脑中动脉闭塞
（MCAO）1 h。术后，Sham 组和MCAO 组即刻腹腔注射生理盐水（0.1 mL/kg），MCAO+GAS各组注射不同剂量天麻素，每24 小时
重复给药1 次，连续7 天。最后一次注射24 h后，对各组小鼠的神经功能进行评分，之后处死动物取脑组织，通过HE、免疫荧光染
色以及Western Blot 观察和比较各组小鼠海马形态学和DCX的表达水平。结果：（1）术后第7 天，MCAO+Vehicle 组小鼠神经功
能评分（3.2± 0.63）显著高于假手术组、MCAO+GAS（M）（1.8± 0.63）和MCAO+GAS（H）组（P<0.05）。（2）术后第7 天，与假手术组
相比较，MCAO+vehicle 组海马颗粒细胞排列不规则，核稍大且固缩深染，齿状回部有较多空洞；与MCAO + vehicle 组比较，
MCAO+GAS（H）组海马空洞样改变减少，细胞排列较整齐，胞膜较完整，核结构较清晰。（3）术后第7 天，与假手术组相比较，DCX
染色阳性细胞数显著减少，而MCAO+GAS（M）组DCX染色阳性细胞数（183± 64.5）和MCAO + GAS（H）组DCX染色阳性细胞
数（195± 93.68）显著高于MCAO+Vehicle 组。MCAO+Vehicle 组海马的DCX 表达水平显著低于假手术组及MCAO+GAS（M）组
和GAS（H）组（P<0.01）。结论：天麻素可能通过上调海马DCX 的表达水平，调节海马神经发生，进而对缺血性脑卒中后再灌注发
挥神经保护作用。 |
| 英文摘要: |
| Objective:To observe the effects of different concentrations of gastrodin (GAS) on the neural function and the
expression of DCX in the hippocampus after cerebral ischemia-reperfusion in mice.Methods:50 male C57BL/6J mice were randomly
divided into five groups: Sham, MCAO+Vehicle, MCAO+GAS (L, L=10 mg/kg), MCAO+GAS (M, M=50 mg/kg), MCAO+GAS (H,
H=100 mg/kg). The mice in Sham group received fake surgery, while the mice in MCAO and MCAO+GAS group were exposed to
MCAO. Mice were injected intraperitoneally with saline or different doses of GAS 1 h after surgery once daily for 7 consecutive days,
and then the neural function scores were assessed before execution. The hippocampal structure was observed by hematoxylin-eosin
staining and immunohistochemistry, and the expression of DCX was also detected byWestern blotting.Results:(1) The neural function
score in MCAO+Vehicle group (3.2± 0.63) was higher than that of Shamand MCAO+GAS (M) (1.8± 0.63) or MCAO+GAS (H) group
on the 7th day after reperfusion (P<0.05). (2) Compared to Sham group, hippocampal granule cell layer was arranged irregularly in
MCAO+Vehicle group on the 7th day after reperfusion, there also appeared pyknosis and void like changes. The void like changes were
reduced and granule cell layer was more clear in MCAO+GAS (H) group in comparison with MCAO+Vehicle group. (3) After
reperfusion, MCAO+Vehicle group showed less number of DCX positive cell in comparison with Sham group. Moreover, MCAO+GAS
(M) group (183± 64.5) and MCAO+GAS (H) group (195± 93.68) showed more number of DCX positive cell in comparison with
MCAO+Vehicle group on the 7th day. Consistent with the result of immunohistochemistry, the expression of DCX in MCAO+Vehicle
was significantly less than that of Sham, MCAO+GAS (M) and MCAO+GAS (H) groups (P<0.01).Conclusion:Gastrodin could regulate
neurogenesis by up-regulating the expression of DCX in hippocampus of MCAO mice, reduce its neurologic impairment in a certain does, and show some protect effects of cerebral ischemia. |
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