| 赵小军 于军 赵海康 张兵 陶绍辉.beta-catenin 基因在人骨肉瘤MG63 细胞系的表达变化[J].,2015,15(31):6031-6034 |
| beta-catenin 基因在人骨肉瘤MG63 细胞系的表达变化 |
| Expression of beta-catenin Gene in Human Osteosarcoma Cell Line MG63Detected by FQ-PCR |
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| DOI： |
| 中文关键词: FQ-PCR 人骨肉瘤MG63 细胞 beta-catenin 基因表达 Wnt3a |
| 英文关键词: FQ-PCR Human osteosarcoma MG63 cells beta-catenin Wnt3a |
| 基金项目:国家自然科学基金项目（81071946） |
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| 中文摘要: |
| 目的：观察分析beta-catenin 基因在人骨肉瘤MG63 细胞系的表达变化。方法：培养MG63 细胞株，用不同浓度的Wnt3a 蛋白或
相同浓度、不同时间段的Wnt3a 蛋白刺激液MG63细胞生长，用FQ-PCR在mRNA 水平检测beta-catenin 在MG63 细胞系的表达
变化。培养MG63 细胞株，用相同浓度的Wnt3a 蛋白刺激，比较刺激组与空白对照组的细胞数目的差别。结果：在细胞生长的第
0，1 天两组的MG63 细胞数量对比没有统计学差异（P＞0.05）；而在第2，3，4 天对照组细胞增殖速度较刺激组缓缓，经比较（t=4.
109，3.892，5.215，均P＜0.05）。beta-catenin 基因表达不会因为Wnt3a 的浓度增加而增加。100 ng/mL的Wnt3a 蛋白刺激beta-catenin 基
因表达最高，其他几个浓度的beta-catenin 基因表达对比没有统计学差异（P>0.05）。在100 ng/mL的Wnt3a蛋白刺激下，beta-catenin 基
因表达会随时间延长而增加，6 h时茁-catenin基因表达最高（P<0.05），随后茁-catenin基因表达则没有统计学差异（P>0.005）。结
论：Wnt蛋白对MG63 细胞有正向增加增殖作用。激活Wnt/beta-catenin 信号通路能促进MG63 的细胞增殖。 |
| 英文摘要: |
| Objective:To analyze the change of beta - catenin gene in bone sarcoma MG63 cell line.Methods:MG63 cell lines
were cultured and Wnt3a protein was added to stimulate the cell growth in different concentrations or in the same concentration and different
time periodsbeta - catenin mRNA expression in MG63 cell line was detected by FQ - PCR. Cultivate MG63 cell lines were cultured
and stimulated with the same concentration of Wnt3a protein, and the difference of cell number was compared.Results:On 0, 1st
day of cell growth, the number of MG63 cells between two groups had no statistical difference (P>0.05); on 2nd, 3rd, and 4th day, the
speed of cell proliferation in control group was slower than that of the stimulating group (t = 4.109, 3.892, 4.109, P<0.05). The expression
of beta-catenin mRNA wasn't increased with the concentration of Wnt3a. beta-catenin expression reached the highest under the stimulation
of 100 ng/mL Wnt3a, but beta-catenin expression had no statistical differences under the other concentration of Wnt3a. Under the stimulus
of 100 ng/mL Wnt3a protein, beta-catenin expression was increased with the time, and reached the peak at 6 h, then beta-catenin expression
were no statistically significant.Conclusion:Wnt can positively increase the proliferation of MG63 cells. Activation of
Wnt/beta-catenin signaling pathway can promote MG63 cell proliferation. |
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