| 杨丽美 纪霞 李云霄 高俊杰 贾少丹 肖芝秀.apelin 基因rs2235306 位点多态性与哮喘的相关性研究[J].,2015,15(30):5850-5853 |
| apelin 基因rs2235306 位点多态性与哮喘的相关性研究 |
| Association between Polymorphismof Apelin Gene rs·2235306 andSusceptibility to Asthma |
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| DOI： |
| 中文关键词: apelin 哮喘 基因多态性 基因频率 |
| 英文关键词: Apelin Asthma Gene polymorphism Genotypes Gene frequency |
| 基金项目:青岛市市南区发展基金项目(2012-5-022-YY) |
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| 中文摘要: |
| 目的：探讨apelin 基因rs2235306 位点多态性与哮喘的相关性。方法：以外周血全血DNA为模板，应用四引物扩增受阻突变
体系PCR（Tetra - primer ARMS PCR, T-ARMS-PCR）方法对158 例哮喘患者（AS）和79 例健康个体（NC）apelin 基因rs2235306 位
点基因型进行分析，同时进行肺功能检查(FEV1、FVC、FEV1/ FVC )。结果：AS 组和NC 组apelin 基因rs2235306 位点等位基因T
和C 频率分布具有统计学意义(X2=6.906，P=0.009，OR=1.688，95%CI=1.140-2.497)，AS 组C等位基因频率显著高于健康对照组；
AS 组和NC 组基因型分布具有统计学意义(X2=14.243，P=0.000，OR=3.894，95%CI=1.861-8.149)，其中CC 基因型患哮喘的风险
较高，为TT+TC基因型的3.894 倍。AS轻度组和AS中重度组基因型CC 和TT+TC 频率及等位基因T 和C 频率比较均无统计
学意义。结论：apelin 基因rs2235306 位点多态性和哮喘的发病具有一定的相关性，C 等位基因可能是哮喘的遗传易感基因，CC基
因型携带者哮喘的患病风险可能增加，但与哮喘的严重程度无明显相关性。 |
| 英文摘要: |
| Objective:To investigate the relationship between apelin gene rs2235306 and asthma.Methods:The genotypes of
apelin were analyzed by tetra - primer amplification refractory mutation system PCR (Tetra - primer ARMS PCR,T-ARMS-PCR)in 158
asthma patients and 79 healthy volunteers. At the same time, we measured the lung function (FEV1, FVC, FEV1/ FVC).Results:There
was significant difference in frequency distribution of apelin genotype between the asthma group and health group (X2=6.906, P=0.009,
OR=1.688, 95 % CI=1.140-2.497). The CC genotype was associated with a 3.894 fold increased risk for asthma than the TT+TC
genotype (X2=14.243, P=0.000, OR=3.894, 95% CI=1.861-8.149). In Mild AS group and Mod-SevereAS group, genotype and allele
frequency had no statistical significance.Conclusion:apelin gene polymorphism rs2235306 was significantly correlated with asthma, the
C allele might be the genetic factor that contribute to individual susceptibility for asthma, the CC genotype carriers may be at increased
risk of asthma, but it has nothing to do with the severity of asthma. |
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