| 齐欣 卢弘 余烁 于晋懿 毛毳.冰片提取物对沙眼衣原体感染HeLa细胞CT703 及CT259 表达的影响[J].,2015,15(29):5645-5647 |
| 冰片提取物对沙眼衣原体感染HeLa细胞CT703 及CT259 表达的影响 |
| Effect of Borneol Extraction on the Expressions of CT703 and CT259 ofHeLa Cells Infected with Chlamydia Trachomatis |
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| DOI： |
| 中文关键词: 冰片提取物 沙眼衣原体 基因 |
| 英文关键词: Borneol extract Chlamydia trachomatis Gene expression |
| 基金项目:北京市自然科学基金项目(7052023) |
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| 中文摘要: |
| 目的：探究冰片提取物对沙眼衣原体感染后的HeLa 细胞模型CT703 和CT259 表达的影响。方法：将成功建立的40 例感染
L2血清型沙眼衣原体的人宫颈癌上皮（HeLa）细胞模型随机分成A、B 两组，分别添加冰片提取物和等剂量生理盐水。观察感染后
HeLa 细胞模型的包涵体数目及大小、RNA 抽提结果完整性以及CT70 与CT259 的表达变化。结果：染色后的40 例受沙眼衣原体
感染的HeLa 细胞模型体内均发现包涵体，在同一时间点B 组细胞内包涵体比A 组大，且数目比A 组多（P<0.05）；两组提取的总
RNA的OD 值均在1.8~2.0 之间，通过RNA 凝胶电泳结果可清楚发现28S、18S 及5S 条带；同一时间点CT259 和CT703 扩增产
物的平均灰度值比较，A 组感染后HeLa 细胞模型样本基因表达量低于B 组，差异具有统计学意义（P<0.05）。结论：冰片提取物能
够有效降低经沙眼衣原体感染的HeLa 细胞模型中CT703 与CT259 基因表达量。 |
| 英文摘要: |
| Objective:To explore the effect of borneol extract on the expressions of CT703 and CT259 in HeLa cell which infected
by Chlamydia trachomatis .Methods:The 40 human cervical epithelial cells which were infected by chlamydia trachomatis were randomly
divided into two groups (group A and group B). The borneol extract and physiological saline were add to the two groups, respectively.
Then the size and number of inclusion bodies, RNA extraction results and the expressions of CT703 and CT259 were observed and analyzed.Results:The cell inclusion bodies were found in 40 HeLa model; At the same point in time, the inclusion bodies in group B were
bigger than those of group A, and the number of inclusion bodies was more than that of groupA (P<0.05). OD value of the extraction of
total RNA was during 1.8 and 2.0 in the two groups, and 18 s, 28 s and 5 s stripe could be clearly found. At the same time, the level of
gene expressions in group A was lower than that in groupB (P<0.05).Conclusion:Borneol extract can effectively reduce the gene expressions
of CT703 and CT259 in the HeLa model infected by chlamydia trachomatis. |
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